琢- 硫辛酸对SD 大鼠下丘脑- 垂体- 肾上腺皮质轴 - 南方医科大学学报.PDF

窑664 窑 南方医科大学学报(JSouthMedUniv) 2010;30(4) 琢-硫辛酸对SD大鼠下丘脑-垂体- 肾上腺皮质轴形态和功能的影响 卜 乐袁刘志民(上海长征医院内分泌科袁上海 200003) 摘要院目的研究琢-硫辛酸对正常和糖尿病大鼠下丘脑-垂体- 肾上腺皮质功能和超微结构的影响遥 方法应用放射免 疫法分别观察了低尧中尧高剂量渊1尧20尧100mg/kg冤琢- 硫辛酸(100mg/kg)连续腹腔注射3 周袁对 SD 大鼠血浆CRH尧 ACTH尧皮质酮水平的影响遥 并运用电镜观察用药前后大鼠垂体尧肾上腺皮质超微结构的变化遥 结果 正常和糖尿病大 鼠低中高剂量琢-硫辛酸处理组CRH水平与相应对照组相比显著降低曰 正常大鼠低中高剂量硫辛酸处理组ACTH 水 平比相应对照组明显降低渊P0.05冤曰正常大鼠低中高剂量琢-硫辛酸处理组和糖尿病大鼠中高剂量琢-硫辛酸处理组皮 质酮水平比相应对照组显著降低 渊P0.05冤遥 琢-硫辛酸处理对正常大鼠下丘脑-垂体- 肾上腺皮质超微结构无明显影 响遥糖尿病大鼠垂体细胞和肾上腺皮质细胞出现功能受抑制状态袁琢-硫辛酸处理21d后垂体促肾上腺皮质细胞和肾上 腺皮质细胞代谢较处理前明显活跃遥 结论琢-硫辛酸能在不同层次直接或间接地发挥对正常和糖尿病大鼠HPA 轴的 抑制作用遥 关键词院琢-硫辛酸曰HPA轴曰超微结构曰放射免疫曰透射电镜 中图分类号院Q45 文献标识码院A 文章编号院1673-4254(2010)04-0664-04 Effectsofalphalipoicacidonhypothalamus-pituitary-adrenalaxisinrats BULe,LIUZhi-min DepartmentofEndocrinology,ChangzhengHospital,Shanghai200003,China Abstract: Objective To study the functional and ultramicrostructural effect sof alpha lipoic acid on hypothalamus-pituitary-adrenal(HPA) axisinnormalanddiabeticrats. MethodsUsingradioimmunoa ayweobservedthe effectsofthreedoses(1,20,and100mg/kg)ofalphalipoicacidinjectedintraperitoneallyfor3weeksontheplasmalevelsof CRH, ACTHandCORinnormalanddiabeticrats. Theultramicrostructuralchangesofthehypophysisandpituitarygland afteralphalipoicacidtreatmentwereobservedundertransmi ionelectronmicroscope. Results Comparedwiththecontrol group,CRHlevelinlipoicin-treatednormalanddiabeticratswa ignificantlyreduced(P0.05).ACTHlevelofthe3lipoicin dosesgroupsofnormalratsdecreased, andasignificantreductionoccurredinmedium-doselipoicingroupofdiabeticrat (P0.05). COR level showed the same change sa sCRH level in normal rats, but decreased significantlyin high- and medium-doselipoicingroupsofdiabeticrats.Lipoicintreatmentproducednoapparenteffectontheultramicrostructuresofthe hypophysisandpituitaryglandcells, whichwerethetargetsofdiabeti