03_引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 　33 ( ) ( ) 5 :396400 2003 引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种 陈永芳 , 郭坚华 , 方中达 (南京农业大学植保系 ,农业部病虫监测与治理重点开放实验室 , 南京 210095) 摘要 : 1995 年在内蒙古临河市新发现了糖甜菜细菌性叶斑病 ,从病斑所分离的 10 个细菌菌株经柯赫氏法则验证 ,均确系该病的 病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析、DNA G+ C mol %和DNADNA 同源性测定进行了鉴定 ,并与植物病原棒形细菌 15 个标准菌株进行了比较。该病原菌为革兰氏阳性细菌 ,不规则短杆状 ,有一 根鞭毛、亚极生或侧生 ,结合其生理生化特性、细胞化学成分和DNA G+ C mol %和DNADNA 同源性测定结果 ,认为应属于短小杆 ( ) ( ) 菌属 Curtobacterium 的萎蔫短小杆菌 Cur. flaccumfaciens ,数值分析也支持这一结论。此外 ,据血清学反应结果及其对短小杆菌 属的其它植物寄主的致病情况 ,认为该病原菌应是萎蔫短小杆菌种下的一个新的致病变种 ,定名为 Curtobacterium flaccumfaciens ( ) pv. beticola pv. nov. Chen et al . , 2000 萎蔫短小杆菌糖甜菜致病变种 。 关键词 : 糖甜菜 ; 细菌性叶斑病 ; 鉴定 ; 萎蔫短小杆菌 ; 致病变种 A ne w pathovar of Curtobacterium flaccumfaciens causing bacterial leaf spot of sugar beet (Nanjing Agricultural University , Nanjing 210095 , China) CHEN Yongfang , GUO Jianhua , FANG Zhongda 　 Abstract : Bacterial leaf spot of sugar beet ( Beta vulgaris var. saccharifera) was found in Linhe City of Inner Mongolia in 1995. Ten bacterial isolates were identified by the means of classical methods , numerical analysis , cellular composition analysis , DNA G + C mol % and DNADNA homologous detection. The results showed that the characteristics of the pathogen were consistent with those of Curtobacterium flaccumfaciens . According to the serological reaction as well as the pathogenicity of the isolates to other plant hosts of the genus , the pathogen is considered to be a new pathovar of Cur. flac cumfaciens . The name is suggested as Curtobacterium flaccumfaciens pv. be