L-肉毒碱联合前列舒通治疗AsAb阳性雄鼠实验探究.docVIP

L-肉毒碱联合前列舒通治疗AsAb阳性雄鼠实验探究【摘 要】 目的:观察L-肉毒碱联合前列舒通对AsAb阳性雄性小鼠的治疗效果。方法：用同种精子及福氏佐剂免疫昆明种雄性小鼠，采用酶联免疫吸附测定法（ELISA）验证制得AsAb阳性动物模型即动物模型组，同时设立空白对照组。动物模型组分别给予L-肉毒碱加前列舒通、L-肉毒碱、前列舒通、强的松、生理盐水，灌胃四周。通过酶标仪测定光密度值(optical density,OD值)观察药物治疗对血清AsAb的影响；通过伟力全自动电脑精子质量分析仪测定精子密度、活率及直线运动速度，并进行统计分析。结果：治疗后OD值测定比较：联合治疗组、L-肉毒碱组、前列舒通组、强的松组OD值均低于生理盐水组，差别有统计学意义（P＜0.05）；L-肉毒碱组OD值高于联合治疗组、前列舒通组及强的松组，差别有统计学意义（P＜0.05）；联合治疗组OD值虽低于前列舒通组和强的松组，但尚不能认为其差别有统计学意义（P＞0.05）；前列舒通组和强的松组相比差别无统计学意义（P＞0.05）。精子参数测定显示：L-肉毒碱组与前列舒通联合治疗组的精子各项参数要优于L-肉毒碱组、前列舒通组、强的松组及生理盐水组，差别有统计学意义（P＜0.05），但与空白对照组比较差别无统计学意义（P＞0.05）；动物模型组中四个治疗组精子相关参数与生理盐水组比较均有不同程度的改善，差别有统计学意义（P＜0.05）； L-肉毒碱组和前列舒通组分别与强的松组相比较其精子参数要好于强的松组，差别有统计学意义（P＜0.05）；前列舒通组精子参数要好于L-肉毒碱组，但无统计学意义（P＞0.05）；生理盐水组精子参数三项均低于空白对照组，差别有统计学意义（P＜0.05）。结论：AsAb不仅影响精子各项参数，还可能通过其它途径降低雄性小鼠的生育力；L-肉毒碱联合前列舒通治疗AsAb阳性雄性小鼠有确切的疗效，为治疗男性免疫性不育提供了一种新方法及翔实、可靠的实验依据。 【关键词】 AsAb阳性; 抗精子抗体； 雄性小鼠 Experiment study on L-carnitine plus QianLieShuTong in treating AsAb positive male mice GAO Guo， XU Jixiu， LIU Lei， GUO Qiang Department of Urology, The First Affiliated Hospital of Shanxi Medical University.Taiyuan 030001, China 【Abstract】 Objectives: To explore the efficacy of L-carnitine plus QianLieShuTong in treating immunological infertility of male mice. Methods: With the same kinds of sperm and Freunds adjuvant immunization Kunming male mice, ELISA method validation to obtain animal models of immune infertility, while the establishment of the normal control group; were given L-carnitine plus QianLieShuTong, L-carnitine, QianLieShuTong, Prednisone, normal saline, by gavage, in one month; Optical density values measured by microplate reader (optical density, OD value) observed drug treatment effects on serum AsAb; Weili Automatic Computer sperm analysis to measure the sperm density, linear velocity and live rates, and statistical analysis. Results: Determination of OD values compared: combined treatment group, L-carnitine group, Qianlieshutong group, prednisone group OD values were lower than the sali