Information on the FESEM (Fieldemission （信息对其场发射）.pdf

Information on the FESEM (Field-emission Scanning Electron Microscope) Radboud University Nijmegen Researchers in biology, chemistry and physics employ the field emission scanning electronen microscope (FESEM) to observe small structures (as small as 1 nanometer = one billion of a millimeter!) on the surface of cells and material. A few examples of object that are studied with a FESEM in practice are organelles and nuclei of cells, synthetical polymeres and coatings of microchips. From your computer you can connect to the simulation FESEM (www.vcbio.science.ru.nl/fesem ) and take a look online at five objects. It is possible to operate a number of interactive settings (e.g. magnification, focus and contrast) in the virtual FESEM, exactly like in the real microscope at the Department of General Instrumentation. Before starting you may need to download and install plug-ins on your computer the first time. You will be guided step by step through the download procedure. Here below you will find information on the functioning of a FESEM: 1. Principle 2. Preparation 3. Source of electrons 4. Column 5. Object chamber 6. Image formation 7. Contact and links 1. Principle 1.1.What does the word FESEM mean? FESEM is the abbreviation of Field Emission Scanning Electron Microscope. A FESEM is microscope that works with electrons (particles with a negative charge) instead of light. These electrons are liberated by a field emission source. The object is scanned by electrons according to a zig-zag pattern. 1.2. What can be done with a FESEM? A FESEM is used to visualize very small topographic details on the surface or entire or fractioned objects. Researchers in biology, chemistry and physics apply this technique to observe structures that may be as small as 1 nanometer (= billion of a millimeter). The FESEM may be employed for example