实时荧光定量PCR 法检测十字花科细菌性黑斑病菌 - 植物保护学报.PDF

妞嶇墿淇濇姢瀛︽姤 锛綇锝曪綊锝庯絹锝 锝忥絾 锛帮綄锝侊綆锝 锛帮綊锝忥綌锝咃絻锝旓綁锝忥綆锛岋紥锛愶紤锛栵紝锛旓紦锛堬紨锛夛細锛曪紩锛欙紞锛曪紪锛 锛わ集锛╋細锛戯紣矶帮紤锛擄紭锛愶紥锛 锝婏紟锝冿綆锝嬶綁锛庯綒锝楋絺锝堬綐锝傦紟锛掞紣锛戯紪矶帮紣锛旐栋锛愶紣锛 瀹炴椂鑽у厜瀹氶噺锛帮迹锛叉硶妫€娴嬪崄瀛楄姳绉戠粏鑿屾€ч粦鏂戠梾鑿 锛 锛 锛掆垪 锛撯垪 鐜嬮亾娉姐€€ 寮犺帀涓姐€€ 闄朵腑浜 銆€ 璋㈠叧鏋 锛堬紤锛庢禉姹熺渷鏉窞甯傛淇濆湡鑲ユ€荤珯锛屾澀宸烇紦锛戯紣锛愶紥锛愶紱锛掞紟婀栧窞甯傚嚭鍏ュ妫€楠屾楠屽眬锛屾禉姹 婀栧窞锛擄紤锛擄紣锛愶紣锛 锛擄紟娴欐睙澶у妞嶇墿淇濇姢绯伙紝鏉窞锛擄紤锛愶紣锛曪紭锛 鎽樿锛氫负鏈夋晥闃叉帶鎴戝浗鐨勬鐤€ф湁瀹崇敓鐗╁崄瀛楄姳绉戠粏鑿屾€ч粦鏂戠梾鑿岋及锝擄絽锝曪絼锝忥綅锝忥綆锝侊綋 锝擄綑锝掞綁锝庯絿锝侊絽 锝愶綎锛 锝嶏絹锝冿綍锝岋綁锝冿綇锝岋絹鍦ㄥ浗鍐呯殑浼犳挱涓庤敁寤讹紝閫氳繃璁捐锛戝鐗瑰紓鎬у紩鐗╋紦锛曪紦锛欙紝鍒╃敤锛戯紦锛掓牚闈舵爣鍜岄潪闈舵爣鑿屼负妯 鏉胯繘琛岋及锛ｏ疾鎵╁锛屽缓绔嬩簡瀹炴椂鑽у厜瀹氶噺锛帮迹锛 娉曪紝骞惰繘琛屼簡妯℃嫙绉嶅瓙甯﹁弻璇曢獙銆 缁撴灉鏄剧ず锛屽紩鐗 锛擄紩锛擄紮 涓哄彧閽堝鍗佸瓧鑺辩缁嗚弻鎬ч粦鏂戠梾鑿屾墿澧炲嚭鐨勭壒寮傛€т骇鐗╋紱鍦ㄦā鎷熺瀛愬甫鑿屾娴嬩腑锛屽父瑙勶及锛ｏ疾 锛 锛 锛 瀵硅弻鎮恫鐨勬娴嬮檺涓猴紤锛 锛ｏ鸡锛碉紡 锝嶏棘锛屽疄鏃惰崸鍏夊畾閲忥及锛ｏ疾 鐨勬娴嬮檺涓猴紤锛 锛ｏ鸡锛碉紡 锝嶏棘锛屽叾涓紤锛 锛ｏ鸡锛碉紡 锝嶏棘 锛 鑿屾恫鐨勶迹锝斿€兼渶浣庯紝涓猴紥锛掜栋锛欙紣锛岋紤锛 锛ｏ鸡锛碉紡 锝嶏棘鑿屾恫鐨勶迹锝斿€兼渶楂橈紝涓猴紦锛曧栋锛楋紦锛屼笖涓嶅悓娴撳害鑿屾恫闂寸殑锛ｏ綌鍊煎潎 鏈夋樉钁楀樊寮傦紱涓嶅悓甯﹁弻鐜囨ā鎷熺瀛愮殑妫€娴嬬粨鏋滆〃鏄庯紝甯歌锛帮迹锛插拰瀹炴椂鑽у厜瀹氶噺锛帮迹锛 鑳芥娴嬪埌鐨勫甫 鑿岀巼鍒嗗埆涓猴紣矶帮紩锛呭拰锛愴栋锛戯紖銆 鐮旂┒琛ㄦ槑锛屽疄鏃惰崸鍏夊畾閲忥及锛ｏ疾娉曚笉浠呭彲鐢ㄤ簬鐥呯鐨勬娴嬶紝涔熷彲鐢ㄤ簬鐥 瀹崇殑鏃╂湡璇婃柇銆 鍏抽敭璇嶏細鍗佸瓧鑺辩榛戞枒鐥呰弻锛涘父瑙勶及锛ｏ疾锛涘疄鏃惰崸鍏夊畾閲忥及锛ｏ疾锛涚瀛愭娴 锛わ絽锝栵絽锝岋綇锝愶綅锝咃綆锝 锝忥絾 锝 锝掞絽锝侊綄鈦冿綌锝夛綅锝 锝戯綍锝侊綆锝旓綁锝旓絹锝旓綁锝栵絽 锛帮迹锛 锝侊綋锝擄絹锝 锝嗭綇锝 锝旓綀锝 锝擄綈锝咃絻锝夛絾锝夛絻 锝勶絽锝旓絽锝冿綌锝夛綇锝 锝忥絾锛帮綋锝咃綍锝勶綇锝嶏綇锝庯絹锝 锝擄綑锝掞綁锝庯絿锝侊絽 锝愶綎锛 锝嶏絹锝冿綍锝岋綁锝冿綇锝岋絹 锝夛綆 锝冿綊锝曪絻锝夛絾锝咃綊锝 锛 锛 锛掆垪 锛撯垪 锛凤絹锝庯絿 锛わ絹锝忥綒锝 銆€ 锛猴綀锝侊綆锝 锛綁锝岋綁 銆€ 锛达絹锝 锛猴綀锝忥綆锝囷綑锝曪綆 銆€ 锛革綁锝 锛э綍锝侊綆锝岋綁锝 锛堬紤锛庯绩锝侊綆锝囷綒锝堬綇锝 锛帮綄锝侊綆锝 锛帮綊锝忥綌锝咃絻锝旓綁锝忥綆 锛 锛︼絽锝掞綌锝夛綄锝夛綒锝咃綊 锛筹綌锝侊綌锝夛綇锝庯紝锛絹锝庯絿锝氾綀锝忥綍锛擄紤锛愶紣锛掞紣锛岋己锝堬絽锝婏綁锝侊綆锝 锛帮綊锝忥綎锝夛綆锝冿絽锛岋迹锝堬綁锝庯絹锛 锛掞紟锛綍锝氾綀锝忥綍 锛ワ綆锝旓綊锝欌亙锛ワ綐锝夛綌 锛╋綆锝擄綈锝咃絻锝旓綁锝忥綆 锝侊綆锝 锛憋綍锝侊綊锝侊綆锝旓綁锝庯絽 锛綍锝掞絽锝侊綍锛岋绩锝曪綒锝