UPLC-MSMS检测果蔬饮料中痕量吡虫啉.PDFVIP

食品研究与开发 ２０17 年 7 月 标准与检测 Ｆood Research And Development 第 38 卷第 13 期 169 DOI ：10.3969/j .issn.1005-6521.2017.13.036 UPLC-MS/MS检测果蔬饮料中痕量吡虫啉 王新梅 （琼台师范学院，海南 海 口57 1127 ） 摘 要 ： 建立一种快速、 有效的超 高效液相 色谱- 串联质谱 法测定果蔬饮料 中痕量吡 虫啉农药残 留的方法。 样品经 0.1 %醋酸- 乙腈提取后 ，采用氨基 固相萃取 小柱进行 净化和 富集。采用 Waters Atalantis T3 色谱柱（2.1 mm×150 mm ， 3 μm ）分 离，以 0.1 % 甲酸- 乙腈为流动相 的梯度洗脱模式下，吡 虫啉标准在 0.5 ng/mL~50.0 ng/mL 浓度 范围内线性 良 好 ，相关 系数 r2 为 0.999 ，检 出限为 0.15 μg/kg ，定量限为 0.50 μg/kg ，加标 回收率达到 93.2 %~96.9 %。 关键词 ： 超 高效液相 色谱- 串联质谱 ；吡 虫啉 ；农药残 留 Determination of Trace Imidaeloprid in Fruit and Vegetable Drink by UPLC-MS/MS WANG Xin-mei （Qiongtai Normal University ， Haikou 57 1127 ， Hainan ， China ） Ａｂｓｔｒａｃｔ ： A rapid and effective method was established for the determination of trace imidaeloprid pesticide residue in fruit and vegetable drink by ultra performance liquid chromatography tandem mass spectrometry （UHPLC-MS/MS ）. After the sample was extracted by 0.1 % acetic acid-acetonitrile ， it concentrated and puri－ fied by amino solid phase extraction column. The separation of targeted compound was performed on a Waters Atalantis T3 chromatographic column （2.1 mm×150 mm ， 3 μm ）using 0.1 % formic acid-acetonitrile as mobile phase under gradient elution mode. The linear range of imidaeloprid was in the range of 0.5 ng/mL-50.0 ng/mL with a correlation coefficient of 0.999. The detection limit was 0.15 μg/kg and the quantitative limit was 0.5 μg/kg. The recovery rate was 93.2 %-96.9 %. Ｋｅｙ ｗｏｒｄｓ ：ultra performance liquid chromatography tandem mass spectrometry ；imidaeloprid