材料与方法 - 中国实验动物学报.DOCVIP

正常小鼠冠状动脉的超声成像技术分析 杨海明1，杜忠东1，上官文1，张艳兰1，董伟2，宋铭晶2，郑枝兰3 （1首都医科大学附属北京儿童医院，2中国医学科学院医学实验动物研究所，3美中互利医疗有限公司，北京，100045） 中文摘要 目的 C57BL/6小鼠C57BL/6小鼠于4、8和12周龄时冠状动脉的情况进行观察。超声心动图显示左冠状动脉内径值冠状动脉内径值左冠状动脉内径值冠状动脉内径值左冠状动脉内径值冠状动脉内径值小【关键词】 超声心动图；小鼠；冠状动脉 【中分类号】P29 Analysis of coronary artery imaging in healthy mice with Echocardiography YANG Hai-ming1, DU Zhong-dong1, SHANG Guan-wen1, ZHANG Yan-lan1, DONG Wei2, SONG Ming-jing2, ZHENG Zhi-lan3 (1Department of Cardiology, Beijing Children’s Hospital, Capital Medical University,2Chinese Academy of Medical Sciences＆Comparative Medical Center, 3Chindex Medical limited company) Abstract Objective To assess the feasibility of coronary artery in C57BL/6 mice With a high frequency ultrasound system and to provide evidence for preparation the murine model of KD. Methods Totally 20 mice were chosen and Visualsonics Vevo 770 UBM was used in this study. On weeks 4, 8 and 12, the developmental changes and dimensions of coronary artery of the mice were measured by echocardiography. Results By echocardiography, the present study successfully detected coronary artery diameter in the murine model of KD. On week 4, the diameter of left coronary artery in the mice was 0.32±0.14 mm, and the diameter of right coronary artery was 0.37±0.02 mm, On week 8, the diameter of left coronary artery in the mice was 0.38±0.06 mm, and the diameter of right coronary artery was 0.37±0.02 mm; on week 12, the diameter of left coronary artery in the mice was 0.38±0.02 mm, and the diameter of right coronary artery was 0.39±0.03 mm. Conclusions The present study successfully detected coronary arteries by echocardiography, and the diameter of coronary artery in the mice could be measured which way be benefit for the study in the murine model of coronary arteries disease. Key words: Echocardiography; murine; coronary artery 前 言 冠状动脉损伤存在多种疾病过程中，有关冠状动脉病变过程中血管结构和功能的研究对冠状动脉病变诊断、治疗 、预防及病因探索具有重要意义。 近年来，C57BL/6小鼠作为主要的