不同条件延缓未受精卵激活对鱼类细胞电融合的影响_吴滟.pdfVIP

26 　 6 V ol.26 N o.6 　 2006 12 Journal of Zhanjiang Ocean U niversity Dec 2006 吴滟,傅洪拓,龚永生,何新龙,熊贻伟 (, , 214081) 　:、 15、20、30min, , , , , , 。, 5 mg/mL , ()2%, (P0.05); 。2 1 mg/mL , , (P0.05)。, 、 , 。2 mg/m L , 。 :;;;; :Q 83 1+S961 :A :1007-7995(2006)06-0001-04 Effect on Fish Cell Electrofusion by Delayed Activation Time for Unfertilized Oocytes under Different Conditions W U Yan, F U H ong-tuo, GONG Yo ng-sheng, H E Xin-long , X ONG Yi-w ei (Key Open Laboratory of the Ministry of Agriculture Aquatic Animal Genetics and Reproduc- tion and Aquaculture Biology , Freshwater Fisheries Research enter of the hinese Academy of Fishery Sciences, Wuxi 214081, hina) Abstract:Unfert ilized oocyt es of loach Praramisgurnusdabry anus w ere socked in sy nt he tic o var- ian f luid (SOF )and H olf reters solutio n f or 15, 20 o r 30 minutes respectively befo re fert iliza- tion .T he result s show ed t hat SOF eff ective delayed activation time of oocyt es af ter being in w a- ter;and H olf rete rs so lution also had som e effect s, but no t so eff icient as SOF .T he o ocy tes, w hich w ere digested by diff erent concentratio ns of T ry psin, used as recipient.Fusio n ex periment s indicated t hat T rypsin w hose mass concentrat ion is 5 mg/m L in SOF, in com pariso n with the control g ro up (H olf rete r s solut ion ), not iceably raised the development rat e of fused egg s t o blastula st ag e.T he stati st ical analy sis show ed t he diffe rence is o bvio us (P0.05).T he ratio s of fused oocyt es developed t o gastrula and hatching st ag es, how ever, w ere not so different w it h the control g ro up .n addition, w hen t he mass co ncent