高盐度废水处理中优势耐盐菌鉴定与生长特性研究.pdfVIP

30 2 Vol.30 No.2 2009 Journal of Qingdao Technological University ＊ 李海云,于德爽 ,张培玉 ( , 266071 ) 　: 50%, /O 4 , 、16sRN , :nyj1 nyj3 Bacillus, nyj2 rthrobacter, nyj4 lcaligenes.30 ℃,pH 7.5 ,4 30%50% , 70%, , .. :;16sRN ;; :X172　　　:　　　:1673—4602(2009)02—0059—06 Identification and Study on Growth Characteristics of Halotolerant Domesticated in High-Salinity Wastewater ＊ LI Hai-yun, YU De-shuang ,ZH NG Pei-yu (School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China) Abstract:Four strains of bacterium identified as superiority halotolerant were isolated from salt-resistant activated-sludge which was cultivated by /O process in aquatic product pro- cessing wastewater simulated with seawater ratio of 50%. ccording to morphological obser- vation, physiological and biochemical characteristics and 16sRN sequence homology analy- sis, it was showed that both nyj1 and nyj3 should be Bacillus, nyj2 rthrobacter and nyj4 lcaligenes.The experimental results showed, at the temperature of 30 ℃and pH 7.5, when the seawater ratios were 30% and 50%, there is a little difference between specific growth rates and generation times of four bacteria, but when the seawater ratio was 70%, four bacteria with the lowest average specific growth rate and longest generation time were slower in growth.This conclusion is of important and theoretical significance for guiding treatment projects of high-salinity wastewater. Key words:halotolerant;16sRN ;phylogenetic tree;growth characteristic 0 　 ,, :2008—09—22 :; (J06I03); (