响应曲面法优化超声波提取红缘拟层孔菌总三萜工艺.PDF

6 2010, Vol. 31, No. 20 食品科学 ※工艺技术 陈先晖，袁 博，鞠秀云，蒋继宏 * (徐州师范大学 江苏省药用植物生物技术重点实验室，江苏 徐州 221116) 采用响应面法对超声提取红缘拟层孔菌总三萜的工艺进行优化，在对温度、液料比、超声时间和超声功 率单因素试验的基础之上，根据中心组合设计原理采用四因素三水平的响应曲面分析法，通过建立数学模型对各因 素显著性和交互性的作用分析，得到了超声提取红缘拟层孔菌总三萜的最佳工艺条件为提取溶剂 95% 乙醇、提取 温度 70 ℃、液料比26:1(mL/g) 、超声时间39min 、超声功率450W ，此时总三萜的实际提取率为9.79% 。 红缘拟层孔菌；响应曲面；超声波提取；总三萜 Using Response Surface Methodology to Optimize the Ultrasonic-aided Extraction of Total Triterpenoids from Fruit Bodies of Fomitopsis pinicola (Swartz.: Fr.) Karst. CHEN Xian-hui ，YUAN Bo ，JU Xiu-yun ，JIANG Ji-hong* (Key Laboratory of Biotechnology for Medicinal Plant, Xuzhou Normal University, Xuzhou 221116, China) Abstract ：For the optimization of the ultrasonic-aided extraction of total triterpenoids from the fruit bodies of Fomitopsis pinicola (Swartz.: Fr.) Karst., a series of single factor experiments were carried out to explore the effects of temperature, material/ liquid ratio, ultrasonic treatment time and ultrasonic power on the yield of total triterpenoids and four-variable, three-level response surface methodology based on the principle of central composite design was employed to analyze the significance and interactions of the above four factors affecting the yield of total triterpenoids by mathematical modeling. The optimal extraction conditions were determined as follows: extraction solvent, 95% ethanol; extraction temperature, 70 ℃; material/liquid ratio, 26:1; ultrasonic treatment time, 39 min; and ultrasonic power, 450 W. The actual yield of otal triterpenoids was 9.79% under such conditions. Key words ：Fomitopsis pinicola ；response surface methodology ；ultrasonic-aided extraction ；total triterpenoids 中图分类号：R284.2