金属离子介导活性氧引起DNA氧化损伤及机制研究.PDFVIP

23 5 V l. 23, N . 5 2003 9 ACTA SCIENTIAE CIRCUMSTANTIAE Sep. , 2003 :2003) :X5031,R9946 : A DNA 袭著革, 晁福寰, 孙咏梅, 杨丹凤, 张华山, 李官贤, 李 媛 ( , 300050) : , , 11 ( DNA) 8DNA . , Fe2+ Cu2+ Cr3+ DNA , Fe2+ Cu2+ Cr3+ . , V3+ Ni2+ Cd2+ Zn2+ C 2+ Fe3+ , 8OHdG DNA , . DNA Fent n DNA . : ; ; DNA ;8 ; DNA Study on the mechanism of oxidative damage of DNA induced by reactive oxygen species due to metal ions XI Zhuge, CHAO Fuhuan, SUN Y ngmei, YANG Danfeng, ZHANG Huashan, LI Guanxian, LI Yuan ( Insti tute f Health and Envir nmental Medicine, Academy f Military Medical Sciences, Tianjin 300050) Abstract:The xidative DNA damage markers 8hydr xy2de xyguan sine and DNA strand breakage, induced by reactive xygen species due t metal i ns in vitr , were bserved using high perf rmance liquid chr mat graph c upled with electr chemical detecti n and single cell gel electr ph resis. The results sh wed that s me metal i ns, such as Fe2+ , Cu2+ and Cr3+ can mediate reactive xygen species t induce x 2+ 2+ 3+ 3+ 2+ 2+ 2+ 2+ idative DNA damage, and the mediati n ability is Fe Cu Cr , while ther metal i ns, such as V , Ni , Cd , Zn , C and Fe3+ , can n t induce xidative DNA damage, resulting in 8hydr xy2de xyguan sine f rming r DNA strand breakage, but may induce DNA cr sslinks. The difference f the ability t induce xidative DNA damage by metal i ns was riginated fr m the different ability f their catalysing Fent n reacti n and binding with DNA. Keywords:metal i ns; reactive xygen; xidative DNA damage; 8hydr xy2de xyguan sine; DNA strand breakage , . , , . , , Cr3+ 6+ [ 1, 2] 2+ , Cr . , Fe