不同剂量阿霉素致大鼠局灶节段硬化肾病模型的建立与比较.doc

不同剂量阿霉素致大鼠局灶节段硬化肾病模型的建立与比较 马晓红1，2，何立群1，2* （1.)上海中医药大学附属曙光医院上海 20上海 20） 摘要目的：方法：肾切除，观察模型大鼠24小时尿蛋白定量、血清肌酐、尿素氮、肾脏病理的。结果：P0.01）。各阿霉素注射剂量组于1周出现尿蛋白明显升高（P0.01）、2周出现血清肌酐明显升高（P0.01）、4周出现血清尿素氮P0.01），并均呈进行性增高，2周时除3mg/kg组血清尿素氮P0.01，P0.05）；8周时各注射剂量组大鼠24小时尿蛋白定量P0.01 ；r=0.9384，P0.01；r=0.9568，P0.01）。各组大鼠肾脏病理均提示肾小球出现局灶节段硬化表现，且硬化程度随注射剂量的增加而呈加重趋势。结论：。 关键词； 【中图分类号】Comparison of the Focal Segmental Glomurular Sclerosis Nephrosis Rat model with different dosage Adriamycin to induce MA Xiao-hong1,2， HE Li-qun1,2* （1.Shuguang Hospital，Shanghai University of TCM，Shanghai 200021，China； 2.Clinical Key Laboratory of TCM，Shanghai 200021，China） Abstract: Objective To explore the effect of Adriamycin with different doses used for the Focal Segmental Glomurular Sclerosis Nephrosis rat model . Methods The Focal Segmental Glomurular Sclerosis Nephrosis rat induced by left nephrectomy plus intravenous injection with different Adriamycin doses,to observe the effect of different Adriamycin doses on?rat model of?survival rate,24-hour?urinary protein excretion, serum?creatinine,urea?nitrogen, and kidney?pathology.Results the survival rate decreased with the increase of injection dosage in different doses group and survival rate of injection dosage of 4.5mg/kg~6mg/kg was reduced to less than 50% at fourth week and eighth week,survival rate had a significant negative correlation with injection dosage at eighth week（r=0.9045，P0.01）. Each group of injection dosage appeared urinary protein significantly increased at first week（P0.01）, serum?creatinine significantly increased at second week（P0.01）and urea?nitrogen significantly increased at fourth week（P0.01）, and all showed progressive increase, serum?urea?nitrogen of each group is higher than the normal except the 3mg/kg group（P0.01，P0.05）, 24-hour?urinary protein excretion, serum?creatinine and urea?nitrogen had a significant positive correlation with injection dosage at eighth week（r=0.9429，P0.01 ；r=0.9384，P0.01；r=0.9568，P0.01）. Renal pathology of all