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液相色谱法测定乳粉中乳糖方法优化
8 6 Vol. 8 No. 6
2017 6 Journal of Food Safety and Quality Jun. , 2017
*
高 山 , 程 敏, 张洪元, 谢 玄
((), 314000)
摘 要: 目的 GB 5413.5-2010
, , 方法
, 30 mL , 1 mL 3%,
, , 结果
, ,
7%, 结论 ,
, ,
关键词: ; ;
Optimization of determination method of lactose in milk powder by liquid
chromatography
*
GAO Shan , CHENG Min, ZHANG Hong-Yuan, XIE Xuan
(Abbott (Jiaxing) Nutritionals Co.,Ltd, Detection Center, Jiaxing 314000, China)
ABSTRACT: Objective To investigate and optimize sample preparation process of GB 5413.5-2010 “National
food safety standard Determination of lactose and sucrose in foods for infants and young children, milk and milk
products ”, so as to solve the problem of low lactose detection result in infant formula powder, and guarantee the
accuracy of detection for lactose in milk powder. Methods During the preparation of the sample solution, 30 mL
water was used to dissolve the milk powder, and 1 mL 3% acetic acid solution was added to the volumetric flask to
assist protein precipitation. Thus, the proportion of acetonit
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