百麦安神冲剂对失眠大鼠睡眠时相影响.doc

百麦安神冲剂对失眠大鼠睡眠时相影响[摘要] 目的：观察不同剂量的百麦安神冲剂(BMASD)对失眠大鼠睡眠时相的影响。方法：在恒温、恒湿、自动光控和电磁屏蔽实验条件下，采用大鼠皮层脑电描记方法，观察百麦安神冲剂对失眠大鼠睡眠时相的影响。结果：百麦安神冲剂中、低剂量均能显著延长失眠大鼠慢波睡眠Ⅱ期(SWS2)和快动眼睡眠(REMS)，从而减少觉醒时间(W)并延长睡眠总时间(TST)；高剂量亦能显著延长失眠大鼠SWS2期和REMS期，但对W及TST无显著影响；与安神补脑液的对比研究表明，百麦安神冲剂中剂量延长失眠大鼠睡眠SWS2期效果优于低、高剂量及安神补脑液。结论：百麦安神冲剂高中低剂量均能显著延长失眠大鼠的SWS2期和REMS期，百麦安神冲剂中剂量组对失眠大鼠睡眠SWS2期延长效果优于其他三组。 [关键词] 百麦安神冲剂；脑电图；失眠；睡眠时相 [中图分类号] R285.5[文献标识码]A [文章编号]1673-7210（2008）09（a）-016-03 Effects of Baimai Anshen Decoction on the sleep phase of insomnic rats HUO Rong1, ＷANG Bao-zhen2, LI Ting-li1 (1.Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China; 2.Qiqihar Hospital of Traditional Chinese Medicine, Qiqihar 161000, China) [Abstract] Objective: To observe the effects of Baimai Anshen Decoction (BMASD) at different doses on the sleep phases of insomnic rats. Methods: Under the experimental conditions of constant temperature, constant humidity, light automaticly and electromagnetic shielding, the method of cortical EEG in rats was used to observe the effects of BMASD at different doses on the sleep phases of insomnic rats. Results: BMASD at middle and low dose could significantly prolong the SWS2 and REMS, and further decreased the W and prolonged the TST. BMASD at high dose could also prolong the SWS2 and REMS, but had no obvious effect on W or TAT. The effect of BMASD at middle dose on prolonging SWS2 of insomnic rats was better than that of low and high doses as well as Anshenbunao liquid. Conclusion: BMASD at three doses can significantly prolong the SWS2 and REMS, and the effect of the middle dose is better than that of the other three group on prolonging the SWS2 of insomnic rats. [Key words] Baimai Anshen Decoction; Electroencephalogram; Insomnia; Sleep phase 百麦安神冲剂是齐齐哈尔中医院研制并经过多年临床验证的具有改善睡眠作用的医疗制剂，具有益气养阴、清热安神的功效，在中医临床中常用于治疗心阴不足、虚热内扰或气阴两虚、心神失养等所致的失眠症。本实验采用多导睡眠描记技术(PSG)与电刺激诱导大鼠失眠的实验研究方法，通过对脑电图(EEG)的分析，观察百麦安神冲剂对正常及失眠大鼠睡眠时相的影响，为临床应用百麦安神冲剂治疗失眠提供实验依据。 1 材料与方法 1.1 实验材料