超临界CO2萃取辛夷中挥发油工艺探究.docVIP

超临界CO2萃取辛夷中挥发油工艺探究[摘要]目的：确定超临界CO2萃取辛夷挥发油的最优工艺，为工业化生产提供参考。方法：设计正交试验考察影响萃取效果的因素，并进行方差分析，确定影响因素的最优水平和最优组合。结果：确定了超临界CO2萃取辛夷挥发油的最优工艺条件，挥发油的平均含量为(4.4±0.5)% (n=3)。结论：该法具有提取时间短、总收得率及有效组分含量高、利于保存有效成分等特点，在辛夷的提取及质量研究方面有其独特的优点。 [关键词]超临界CO2萃取；辛夷挥发油；含量；正交试验 [中图分类号]R284.2[文献标识码]A [文章编号]1673-7210（2007）08（c）-101-02 Study on the technology of essential oil from biond magnolia flower by supercriticalCO2extraction ZHAI Bao-tong1, LIU Feng-xia2 (1.Nan Yang Central Hospital,Nanyang 473004,China;2.Nanyang Institute of Technology,Nanyang 473004,China) [Abstract]Objective:To define the optimal technology parameters of supercriticalCO2extraction to extract essential oil from biond magnolia flower,and to offer reference to the industrialized produce in future.Methods:Orthogonal design was adopted to inspect the factors,which influenced the effect of extraction.Variance analysis was used to define the optimal level and society.Results:The optimal extracting conditions of essential oil,which was extracted from biond magnolia flower by supercriticalCO2extraction,were selected.The final average content of essential oil was(4.4±0.5)%(n=3).Conclusion:The supercriticalCO2extraction ,which is used to extract essential oil,has many characteristics such as short extraction time,high yield,high content and also in favour of maintaining of active principle.This method has distinct fortes in the research of biond magnolia flower,including the study on the extraction and quality. [Key words]SupercriticalCO2extraction;Biond magnolia flower;Essential oil;Content;Orthogonal design 辛夷的主要化学成分为挥发油，包括桉油精、丁香酚、胡椒酚甲醚(chavicolmethylether)等，有明显的收缩鼻黏膜血管与抗组织胺作用[1,2]，并能增加血流速度、改善微循环及良好的抗过敏和平喘作用[3~6]，可以作为治疗急、慢性鼻窦炎和过敏性鼻炎药物的组分。本实验以挥发油含量为指标，对辛夷挥发油超临界CO2萃取工艺进行了研究。 1 仪器与材料 1.1仪器 超临界萃取设备HA120-50-01型（江苏南通华安超临界萃取有限公司）。 1.2材料 辛夷药材(南阳市药材公司)。 2 方法与结果 2.1 挥发油含量的测定方法 按中国药典2000年版一部附录XD挥发油测定法进行测定。 2.2 正交试验设计及结果 影响辛夷中挥发油提取效果的因素主要有萃取温度、萃取压力、萃取时间和CO2流量等。因此，从上述四个因素出发，按表1设计辛夷中挥发油提取试验的因素水平表