系统性红斑狼疮患者外周血T淋巴细胞Foxp3的表达和转化生长因子-β1水平与病情的相关性.pdf

·592· 生堡匝遑瘟堂苤查2Q螋生2旦箜!≥鲞筮2塑鱼injB量￡女B丝Q!，§!P!￡垡出笪2Q螋：y迅：13，盟Q：! 系统性红斑狼疮患者外周血T淋巴细胞Foxp3的 表达和转化生长因子一13。水平与疴隋的相关性 叶华代琛贤李春王世瑶 栗占国 mRNA和转化生 【摘要】目的探讨系统性红斑狼疮(SLE)患者外周血调节性T淋巴细胞Foxp3 长冈子(TGF)一B。的水平与SLE病情活动的关系。方法对SLE患者采用实时定量聚合酶链反应(PCR) mRNA的表达。对病情活动组患者进行随访．在疾病稳定期再次检测 检测外周血单个核细胞F0xp3 Foxp3mRNA水平并用酶联免疫吸附试验(ELISA)检测疾病缓解前后血浆TGF—B，的浓度。结果病情活 mRNA水平明显低于非活动组(PO．OI)和健康对照组(PO．OI mRNA 动组Foxp3 o在疾病稳定期Foxp3 水平和血浆TGF—B。浓度较活动期明最升高。结论Foxp3表达及TGF—B，浓度与SLE疾病活动呈负相 关，提示调节性T淋巴细胞在SLE发病中有重要作用。 【关键词】 红斑狼疮，系统性；T淋巴细胞；转化生长因子13 The of the of and iscorrelatedwith expressionFoxp3transforminggrowthfactor-ISl activitysystemic YE DA1 lupuserythematosusHuc4 Chen-xian,LIChan，WANG Shi-yao，L1Zhan-guo．Departmentof j 100044，China Rheumatology,People Hospital,PekingUniversity,Beqing author：LI CorrespondingZhan-guo，Email：zgli98@yahoo．con To therelation of bloodand [Abstract】 betweenthe in objecfiveclarify expressionFoxp3peripheral factorbetal the of growth (TGF—B1)an(1 erythematosus(SLE)． plasmatransforming activitysystemiclupus Methods mRNA of28activeSLE inactiveSLE and16 Foxp3 expression patients．13 patients healthy controlswasdetermined chainreaction．ActiveSLE werefollowed mRNA bypolymerase patients up：Foxp3 of20active wasmeasuredinthestablestatusandthe wasmeasured expression patients plasmaTGF—Bt by EUSAintheactiveandstabl