高浓度葡萄糖对培养血管内皮细胞活力和黏附分子表达的影响.pdfVIP

2005 6 Jun. 2005 26 3 Journ l of C pit l University of Medic l Sciences Vol. 26 No. 3 高浓度葡萄糖对培养血管内皮细胞活力 和黏附分子表达的影响 穆王君史斌 ( ) : , - 1( VCA M- 1) - 1( ICAM- 1), , ICAM- 1 , V CAM-1 ICAM- 1VCA M- 1 , : ; ; + : R 322. 1 2 Effects of High Glucose Concentration on Adhesion Molecules Expression and Survival in Cultured Human Umbilical Vein Endothelial Cells M u Jun, Shi Bin ( Dep ar tment of I nter nal M ed ic ine, F ux ing H osp ital , Cap ital Univ ersity of M ed ical Sciences ) ABST ACT: ObjectiveT o investig te whether or not surviv l w s decre sed fter incub tion w ith high glucose concentr tions in culture medi , nd to ex mine t he effects of glucose on the expression of intercellul r dhesion molecule- 1( ICAM- 1) v scul r cell dhesion molecule( V CAM- 1) in cultured hum n umbilic l vein endotheli l cells. MethodsHum n umbilic l vein endot heli l cells ( ECV 304) were incub ted in culture medium with 11. 2 mmol/ L, 16. 8 mmol/ L nd 33. 6 mmol/ L glucoseconcentr tions for 24 h, 48 h, 72 h respectively. T he tryp n-blue exclusion test w s used to study the influence of D-glucose on cell survi l. The expression of intercellul r dhesion molecule- 1( ICAM- 1) , v scul r cell dhesion molecule- 1( VCA M- 1) w ereme sured by flow : 2004-03-25 study. He rt, 2002, 88: 555~ 558 4 [ 6 ] Gosselink A T M , Smit h A J , Crijns G J , et l . [ 1] K neW B , Wolf PA , Benj min E J , et l . Prev lence , Alter tion of peripher l v sodil tory reservec p city inc