板栗总苞化学成分和其抗菌活性.docVIP

板栗总苞的化学成分及其抗菌活性 焦启扬，吴立军，孙博航，黄健，高慧媛* (沈阳药科大学 中药学院 ,辽宁 沈阳 110016) 摘要︰目的 体外抗菌实验结果显示板栗Castanea mollissima Blume总苞的95％(Φ)乙醇提取物具有抗菌活性, 对总浸膏的化学成分进行分离和结构鉴定,。方法 采用硅胶柱色谱、凝胶柱色谱和重结晶等多种分离方法对板栗总苞的95％(Φ)乙醇提取物的化学成分进行分离，并通过理化性质、谱学分析等方法对化合物进行结构鉴定；结果 分离得到18个化合物，分别鉴定为5-羟甲基糠醛(1)、没食子酸 (2)、对羟基苯甲酸 (3)、对苯二酚(4)、麦角甾-6,22-二烯-3β,5α,8α三醇 (5)、莽草酸(6)、豆甾-4-烯-6β-3酮(7)、 Ganschisandrin (8)、齐墩果酸(9)、山柰酚-3-O- (6″-反式-对-香豆酰基) -β-D-葡萄吡喃糖苷（10）、 山柰酚-3-O- (6″,４″- 双-反式-对-香豆酰基)-β-D-葡萄吡喃糖苷(11)、2-羟基-丁二酸二丁酯(12)、槲皮素(13)、大黄素(14), 3′,3-dimethoxylarreatricin(15)、原儿茶酸(16)、乌索酸(17)、山柰酚(18)。结论 板栗总苞的95％乙醇提取物的总浸膏及水层对大肠杆菌、金黄色葡萄球菌和藤黄微球菌均有一定的抑性活性；化合物7、8、9、12为首次从栗属植物中分离得到，化合物6、14为首次从该种植物中分离得到。 关键词：板栗总苞；化学成分；结构鉴定 中图分类号：R 284 文献标识码：A Chemical constituents from the involucre of Castanea mollissima Blume and its anti-bacteria activity JIAO Qi-yang, WU Li-jun, SUN Bo-hang, HUANG Jian, GAO Hui-yuan (School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016 ,China) ABSTRACT: OBJECTIVE The result in vitro showed the inhibition effect on bacteria and to isolate the chemical constituents and elucidate their structures from the involucres of Castanea mollissima Blume. METHODS The compounds were isolated by chromatogrammphy on silica gel columns, Sephadex LH-20 and recrystallizing methods. NMR and TLC were employed for the structural identification. RESULTS Eighteen compounds were obtained and elucidated as hydroxymethylfurfural (1), gallic acid (2), p-hydroxy benzoic acid (3), hydroquinone (4), ergost-6,22-diene-3β,5α,8α-triol (5), shikimic acid (6), stigmast-4-en-6β-ol-3-one (7), Ganschisandrin (8), oleanolic acid (9), kaempferol-3-O- [6-O-(E)-p-coumaroyl]-β-D-glucopyranoside (10), kaempferol-3-O- [6,2-di-O- (E) -p-coumaroyl] -β-D-glucopyranoside (11), 2-hydroxy-1,4-dibutylbutanedioate (12), quercetin(13), emodin(14), 3′,3-dimethoxylarreatricin (15), 3,4-Dihydroxybenzoic acid(16), ursolic acid(17), kaempferol(18) respectively. CONCLUSION The 95% EtOH extraction and water layer showed t