HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素的含量.PDFVIP

HPLC 同时测定小蓟配方颗粒中蒙花苷和芹菜素的含量 何冬黎，张晓春，李倩(湖北医药学院附属随州医院药学部，湖北 随州 441300)  摘要：目的 建立 HPLC 同时测定小蓟配方颗粒中蒙花苷和芹菜素含量的方法。方法 采用 Diamonsil-C18(4.6 mm× 1 250 mm ，5 μm) 色谱柱，以乙腈-1%冰醋酸水溶液为流动相进行梯度洗脱，流速：1.0 mL·min ，检测波长：335 nm ，柱 温：30 ℃。结果 蒙花苷和芹菜素进样量分别在0.3~1.5 μg (r=0.999 4)和0.075~0.375 μg (r=0.999 7) 内与峰面积呈良好的 线性关系；加样回收率分别为99.19%和99.12% ，RSD 分别为 1.27%和 1.26%。结论 该方法操作简便，重复性好，可用 于小蓟配方颗粒中蒙花苷和芹菜素的含量测定。 关键词：小蓟配方颗粒；蒙花苷；芹菜素；高效液相色谱法 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2017)03-0410-03 DOI: 10.13748/ki.issn1007-7693.2017.03.024 Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC HE Dongli, ZHANG Xiaochun, LI Qian(Department of Pharmacy, Suizhou Hospital, Hubei University of Medicine, Suizhou 441300, China) ABSTRACT: OBJECTIVE To develop a method for content determination of linarin and apigenin in Cirsii Herba Dispensing Granule by HPLC. METHODS The separation was carried out on Diamonsil-C18 column (4.6 mm×250 mm ，5 μm) under gradient elution with acetonitrile-1% glacial acetic as mobile phase at a flow rate of 1.0 mL·min1. The detection wavelength was 335 nm and the column temperature was maintained at 30 ℃ . RESULTS There were good linear relationships between the masses and the peak areas of linarin and apigenin in the ranges of 0.31.5 μg (r=0.999 4) and 0.0750.375 μg (r=0.999 7), respectively. The average recoveries were 99.19% and 99.12%, and RSDs were 1.27% and 1.26%. CONCLUSION The method is simple and reliable for the determination of linarin and apigenin in Cirsii Herba Dispensing Granule. KEY WORDS: Cirsii Herba Dispensing Granule; linarin; apigenin; HPLC 小蓟为菊科植物刺儿菜 Cirsium