gdf-9和bmp-15在不同品系小鼠中的多态性和-中国试验动物学报.docVIP

GDF-9和BMP-15在不同品系小鼠中的多态性和生殖能力关联分析 殷昆仑1,2，张长勇2，王天奇2，暴国2，付淑霞2，何嘉玲2，孙德明2 （1.北京协和医学院研究生院，北京 100730；2.国家人口计生委科学技术研究所，北京 100081） 【摘要】目的 研究GDF-9和BMP-15在繁殖能力不同的品系小鼠ICR和BALB/c中的多态性，为筛选相关的模型动物种群提供参考资料。方法 采用直接测序法筛查多态性位点，并对多态性位点构建双荧光素酶报告基因载体,检测5’UTR对下游基因的影响。结果 ICR小鼠的产仔数是BALB/c的2.8倍左右；在ICR和BALB/c之间发现了GDF-9存在三个多态性位点，一个位于5’端的非编码区，另外两个位于蛋白编码区；双荧光素酶检测结果显示GDF-9的5’UTR区可以促进下游基因的表达。BMP15在两种品系中不存在差异性位点。结论 GDF-9在ICR和BALB/c这两个品系小鼠中存在多态性位点，这些位点的变化对下游基因的表达起一定的促进作用。BMP15在ICR和BALB/c小鼠中不存在多态性位点。 【关键词】GDF-9；BMP-15；小鼠；繁殖力；基因多态性；作用机制 Polymorphism identification in mice GDF9 andBMP15 genes and their association analysis with litter size Yin Kun-lun1,2，Zhang Chang-yong2，Wang Tian-qi2，Fu Shu-xia2，Sun De-ming1,2 (1. Graduate School，Peking Union Medical College, Beijing 100730, China；2. National Research Institute for Family Planning, Beijing 100081, China) 【Abstract】Objective To study the polymorphism of GDF-9 and BMP-15 in mice of different strains, different reproductive capacity, for screening related model animal population provide reference material.. Methods Screening polymorphism loci by using direct sequencing method, and testing 5 UTR impact on downstream genes by constructing luciferase dual reporter vector. Result ICR mice litter size is about 2.8 times the BALB/c; In ICR and BALB/c, respectively, found three polymorphisms, one at the 5 end of the non-coding region, the other two located in the protein coding region ; Dual-luciferase test results show that the 5UTR area of GDF-9 can promote the expression of the downstream gene. BMP15 in two strains are not present in the difference site .Conclusions GDF-9 polymorphic loci exist in ICR and BALB/c strains, changes in these sites play a certain role in promoting the expression of the downstream gene. 【Key words】 GDF-9; BMP-15; mice; fecundity; gene polymorphism; mechanism 生长分化因子9 (growth differentiation factor 9，GDF-9) 和骨形态发生蛋白15(bone morphogenetic protein 15，BMP-15)是由卵母细胞分泌的两种生长因子,对哺乳动物的正常生殖能力是不可或缺的[1]。GDF-9和BMP-15均属于转录生长因子β（Transformin