furin抑制剂αpdx对宫颈癌hela细胞生长转移和成瘤的影响.pdfVIP

·432 · doi 10.3969/j.issn. 1673-4254.2015.03.24 J South Med Univ, 2015, 35(3): 432-436 基础研究 FurinFurin抑制剂抑制剂αα11-PDX-PDX对宫颈癌对宫颈癌HeLaHeLa细胞生长细胞生长、、转移和成瘤的影转移和成瘤的影响响 1 2 1 1 1 2 石 冲 ，张国彬 ，韩宝生 ，杨俊红 ，刘 恒 ，习瑾昆 1 2 唐山市妇幼保健院生殖遗传科，河北 唐山 063000 ；河北联合大学研究生学院，河北 唐山 063000 摘要：目的研究α1-PDX对宫颈癌细胞株HeLa 细胞生长、转移及成瘤的影响并探讨其机制。方法 α1-PDX 转染HeLa 细胞，通 过MTT 细胞增殖实验，Boyden 细胞迁移和侵袭实验观察HeLa 细胞的生长,迁移和侵袭能力。采用蛋白印迹法检测细胞Furin 及产物膜性Ⅰ型金属蛋白酶（MT 1-MMP）蛋白量的变化，荧光酶底物实验检测Furin 活性。制作HeLa 细胞致瘤裸鼠模型研究 肿瘤体内生长。结果 细胞增殖实验结果显示，与对照组相比24 h α1-PDX 组HeLa 细胞体外生长抑制18.4% ，差异有统计学意 义（P0.01）；细胞迁移和侵袭实验结果显示，α1-PDX 处理组穿过滤膜和基质胶的HeLa 细胞数量均明显少于对照组（P0.01）； HeLa/PDX 可显著降低Furin 的活性和MTI-MMP 的蛋白水平。HeLa/PDX 致瘤裸鼠的成瘤机率及皮下肿瘤体积明显小于对照 组。结论 α1-PDX 可有效抑制人宫颈癌HeLa 细胞的生长、转移及成瘤能力，机制可能与降低Furin 活性及产物MTI-MMP 的表 达有关。 关键词：宫颈癌；HeLa ；Furin ；膜性Ⅰ型金属蛋白酶；α1-PDX Effects of α1-PDX, a furin inhibitor, on growth, invasion, and tumorigenicity of cervical cancer HeLa cells 1 2 1 1 1 2 SHI Chong , ZHANG Guobin , HAN Baosheng , YANG Junhong , LIU Heng , XI Jinkun 1Department of Reproduction and Genetics, Maternal and Child Health Hospital, Tangshan 063000, China; 2 Graduate School, Hebei United University, Tangshan 063000, China Abstract: Objective To investigate the effects of the furin inhibitor α1-PDX on the growth, invasion, and tumorigenicity of cervical cancer cells and explore the mechanisms. Methods The changes in the growth, migration and invasion of α 1-PDX-transfected HeLa cells were observed using MTT assay, Boyden migration and invasion assay. The protein levels of furin and MT1-MMP were measured using Western blotting and furin activity was detected by enzyme activity assay in the transfected cells. HeLa cells were seeded subcutaneously in