甲醛湿敷法建立犬窦房结损伤模型的试验研究-第三军医大学学报.docVIP

甲醛湿敷法建立犬窦房结损伤模型的实验研究 张志辉，程军，宋治远△ （第三军医大学西南医院心内科 重庆市介入心脏病学研究所 重庆，400038） 探讨甲醛湿敷法建立犬窦房结损伤模型的可行性和电生理特征。方法　20只试验犬随机分为术后2h、术后24h、术后1w及术后4w组（每组5只）。用20%甲醛湿敷窦房结区建立窦房结损伤模型。各组于湿敷前、湿敷后2h以及各自相对应时间点行电生理功能检测，同步记录体表心电图。结果　20只实验犬平均甲醛湿敷时间为6.2±2.6（3～12）min。5/20只犬甲醛湿敷后窦性心率明显减慢（下降35%～45%），无心律失常；4/20只犬除窦性心率明显减慢外出现窦性心律不齐、频发房性早搏，但很快恢复为窦性节律；11/20只犬予甲醛湿敷后P波消失，呈交界性逸搏心律，其中4只于术后24h行体外ECG监测时转为窦性心律，但HR仍显著低于湿敷前。湿敷后2h组、24h组、1w组及4w组的HR均较甲醛湿敷前显著减慢（p0.01）；各组湿敷后的SNRT及SNRTc则均较湿敷前明显延长（p0.01或0.05）。结论　甲醛湿敷法建立犬窦房结损伤模型简便易行，可致窦房结细胞不可逆性损伤，其电生理改变与临床综合征表现相似。 关键词：犬；窦房结；损伤；甲醛；电生理 Research on establishment of sinoatrial node damage model in canine induced by formaldehyde wet dressing Zhang Zhi-hui, Cheng Jun, Song Zhi-yuan (Department of Cardiology, Southwest Hospital, The Third Military Medical University; Interventional Cardiology Institute of Chongqing, Chongqing 400038, China) Abstract：Objective To explore the feasibility and reliability to build a sinoatrial node damage model in canine induced by formaldehyde wet dressing. Method Twenty canine were randomly divided into 4 groups ( 5 in each group): 2h、24h、1w and 4w groups after wet dressing. The sinoatrial node area of canine was damaged by 20% formaldehyde wet dressed. Sinus node function was measured before、2h after wet dressing and corresponding times of each group. Electrocardiogram of body surface were recorded synchronically. Results: Average wet dressing time course was 6.2±2.6（3～12）min。5/20 canine showed significantly decrease of HR by 35%～45% after wet dressing without arrhythmia. Sinus arrhythmia、premature atrial contraction appeared after wet dressing in 4/20 canine, but recovered regular sinus rhythm quickly. In 11/20 canine P wave disappeared after wet dressing and became junctional rhythm. Among of them, 4 canine recovered sinus rytham 24h later with a significantly slower HR than before wet dressing. HRof each group was significantly decreased than before wet dressing（p0.01）while SNRT and SNRTc were increased significantly. Conclusion: Establ