如何提高大鼠子宫内膜异位症模型的建模成功率-中国试验动物学报.doc

提高大鼠子宫内膜异位症建模成功率的新方法研究 李龙卫，王艳云，陈 琦，王亚萍，韩 璐 （大连医科大学附属妇产医院暨大连市妇幼保健院，辽宁 大连 116033） 【摘要】目的 为子宫内膜异位症治疗新方法的研究提供理想的动物模型。方法 取64只性成熟、未孕、动情周期规律的SD大鼠，于动情期在腹膜、皮下利用“刀划法”行自体子宫内膜移植，术后4周比较两移植部位建模情况。结果 大鼠动情期自体子宫内膜移植总体成功率为93.3%，其中腹膜移植成功率为51.7%，皮下移植成功率为88.3%，两者比较差异有统计学意义；比较两部位异位病灶的体积，差异无统计学意义。结论 利用“刀划法”建立大鼠子宫内膜异位症模型成功率较高，皮下移植比腹膜移植成功率高。 【关键词】子宫内膜异位症；动物模型；动情期；啮齿 A new method for improving the success rate of the establishment of endometriosis models in rats LI Long-wei,HAN Lu,WANG Yan-yun （Gyecology Ward,the Maternity Affiliated Hospital of Dalian Medical University,Dalian Maternal and Child Health Hospital,Dalian 116033,China) [Abstract]Objective To provide ideal endometriosis animal models for research new treatment methods. Methods 64 Sexual mature without pregnant SD rats who has regular oestrous cycle were underwent operation that autologous endometrium were transplantated to peritoneum and subcutaneous with lancet gently cut tissue of where to transplant in the rut,and compared the model of peritoneum and subcutaneous after 4 weeks.Results The general success rate of autologous endometrium transplantation in rat estrus was 93.3%,and the peritoneum transplantation success rate was 51.7%,subcutaneous was 88.3%, the difference of the two place transplantation has statistical significance.Compare the two parts of volume of endometriosis,there was no statistically significant difference.Conclusion Using the lancet to establish the endometriosis model has a high success rate,and subcutaneous model is better than peritoneum. [Keywords] Endometriosis; Animal model; Estrus; Rodentia [作者简介]李龙卫（1987-），女，住院医师，硕士，妇产科，E-mail：@163.com。 [通讯作者]韩 璐，女，教授，硕士生导师，E-mail163.com 组织学观察可见：异位病灶与子宫内膜结构基本一致，但间质层偏薄，腺体少，囊泡外周可见少量肌层组织残留（见图2B、C）。紫蓝色结节（图1C）经组织学证实为异位病灶，腔内含中性粒细胞和泡沫细胞。可能与建模时刀划出血有关。 将咬断缝线再次麻醉缝合后死亡的大鼠解剖发现，皮下移植子宫内膜已基本成活，腹膜移植子宫内膜因缝合存在张力，未成活。此后的45只大鼠行建模手术时，移植到腹膜的子宫内膜均采用无张力缝合。 腹膜移植成功31例，成功率51.7%（31/60）；皮下移植成功53例，成功率88.3%（53/60）；两部位建模成功率比较，差异有统计学意义（P0.05）。见表。 表 建模大鼠腹膜移植与皮下移植成功率比较（%） Tab The c