首参颗粒对颈动脉粥样硬化患者端粒端粒酶的影响.doc

首参颗粒对颈动脉硬化患者端粒、端粒酶的影响 楼丹飞1，申定珠2，陈川*，李越华1，沈睿1，(陈久林2，邢三丽2，刘特2 Effects of Chinese herbal medicine Shoushen Granule on telomere、telomerase on patients with carotid atherosclerosis Danfei Lou1,Dingzhu Shen2，Chuan Chen2*，Yuehua Li1，Rui Shen1，Jiulin Chen2，Shanli Xing2，Te Liu2 1 Shanghai Municiple Hospital of TCM，Shanghai，200071 2Shanghai Geriatric Institute of Chinese Medicine，Shanghai，200032 摘要：目的：观察补肾中药复方首参颗粒对颈动脉粥样硬化（atherosclerosis, AS）患者外周血端粒、端粒酶的影响，初步探讨其作用机制。方法：选取156例颈动脉粥样硬化患者，随机分为治疗组83例和对照组73例，对照组口服普他汀，治疗组服用首参颗粒，疗程均为6个月。于治疗前后分别采用（Real-Time Quantitative Polymerase Chain Reaction , RQ PCR）方法检测外周血端粒、端粒酶的变化。：结论：调控颈动脉粥样硬化患者外周血端粒长度及端粒酶活性可能是补肾中药复方首参颗粒有效作用机制之一。 Abstract: Objective: To investigate the effect of Invigorating kidney herbal compound Shoushen Granule on peripheral blood telomere, telomerase in patients with carotid atherosclerosis (AS) .Methods: 156 patients with carotid atherosclerosis, 83 cases were randomly divided into treatment group, 73 cases were control group, the control group was treated with Pravastatin Sodium Tablets, the treatment group was treated with Shoushen Granule, the treatment lasted 6 months. Before and after treatment, Real-Time Quantitative Polymerase Chain Reaction (RQ PCR) was used to detect the peripheral blood of telomere and telomerase changes. Results: after 6 months’ treatment, there were significant differences of telomere length between before and after treatment of traditional Chinese medicine group (P 0.01), there were significant differences of telomere length between before and after treatment of western medicine group (P 0.05), there was no significant difference between Chinese medicine group and western medicine group(P＞0.05).Telomerase activity was improved after treatment than before treatment in both groups(P0.05). Conclusion: The regulation of peripheral telomere length and telomerase activity in patients with carotid atherosclerosis may be one of the mechanisms of Invigorating kidney herbal compound Shoushen Granule.