红豆杉内生真菌次生代谢物的分离鉴定及其抗菌活性分析.PDF

生 物 工 程 学 报 Chin J Biotech 2009, March 25; 25(3): 368-374 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 工业生物技术 1 1 2 1 陈淑娟 , 刘佳佳 , 龚汉祥 , 杨栋梁 1 , 410083 2 , 215500 : 以产紫杉醇类化合物的云南红豆杉内生真菌 12.3.2 为目标菌株, 对其次生代谢物进行分离鉴定和抗菌活性研 究。通过硅胶柱层析, 共分离得到3 个主要成分, 红外光谱、质谱、核磁共振氢谱等光谱解析鉴定其分别为松柏烯、邻 苯二甲酸二异辛酯、油酸乙酯。生物活性试验表明 3 种化合物对金黄色葡萄球菌、枯草杆菌、大肠杆菌、绿脓杆菌和 白色链球菌这 5 种致病菌都有一定的抑制作用。其中松柏烯对金黄色葡球菌、枯草杆菌和白色念珠菌有较强的抑制作 用。松柏烯是首次报道从植物内生真菌次生代谢物中分离得到, 这为松柏烯的来源开辟了一条重要的途径, 同时也为松 柏烯的进一步开发利用奠定了基础。 : 内生真菌, 次生代谢物, 抗菌活性, 松柏烯, 邻苯二甲酸二异辛酯, 油酸乙酯 Identification and antibacterial activity of secondary metabolites from Taxus endophytic fungus 1 1 2 1 Shujuan Chen , Jiajia Liu , Hanxiang Gong , and Dongliang Yang 1 Department of Pharmacy Engineering , College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China 2 Suzhou Changshu Bureau of Quality and Technique Supervision, Changshu 215500, China Abstract: We isolated three secondary metabolites by silica gel column chromatography from endophytic fungus 12.3.2 that was isolated from Taxus yunnanensis and could produce taxanes. They were identified as cembrene (3,7,11-trimethyl-14-(1-methylethyl)-1,3,6,10-cyclotetradecatetraene), diisooctyl phthalate (1,2-benzenedicarboxylic acid diisooctylester) and ethyl oleate (9-octadecenoic acid-ethyl ester) by infrared spectra (IR), mass spectra (MS) and