甘草酸的提取及其抑菌活性研究-湖南大唐生物技术有限公司.pdf

Nat ProdResDev 2006, 18:428-431 :1001-6880(2006)03-0428-04 　 　 ＊ 崔永明,余龙江 ,敖明章,杨　英,王　磊 (, 430074) 　:。, 、 , , 20 0.7% 2, 20 min。, , , , (MIC) :0.0625, 0.125,0.25,0.03125 mg/mL, 。 :;;;; :284.2;Q946.91 :A Studies on Glycyrrhizic Acid Extraction and Its Bacteriostatic Activities ＊ CUI Yong-ming,YU Long-jiang ,AO Ming-zhang,YANG Ying,WANG Lei (College of LifeScience and Technology,Huazhong University of Scienceand Technology,Wuhan 430074, China) Abstract:Comparison of extraction methods for the glycyrrhizic acidwas presented.The experiments showed that ultrasonic-as- sisted extraction (UAE)was faster and more effective than the conventional whisk extraction, enzymatic extraction and repeat freeze-dissolve extraction, and the optimal UAE conditionswere adding 20-fold ammonia (0.7%v/v), extracting for two times, 20 min each time.Further more, the antibiotic bioactivity in vitro of the extracted glycyrrhizic acid was studied.The result showed the glycyrrhizic acid had obvious antibiotic action against such pathogens as Staphylococcus aureus, Escherichia coli, Bacillussubtilis and Bacteriu aeruginosa, and the minimal inhibitory concentration (MIC)were 0.0625, 0.125, 0.25, 0.03125 mg/mL respectively. Key words:Radix glycyrrhiza;glycyrrhizic acid;ultrasonic-assisted extraction;antibiotic activity 　　(Radix glycyrrhiza) :, (Glycyrrhiza uralensis Fisch ) 。 [1] :(Escherichia coli), ,, 。,, (Staphylococcus aureus ), , 。 (Bacillus subtilis ), (Pseudo onas aerugi-