小鼠胎盘源干细胞移植对臭氧致衰老模型的影响.pdf

Medical GuangdongJournal ·404· 广东医学2010年2月第3l卷第4期 小鼠胎盘源干细胞移植对臭氧致衰老模型的影响 李军1，张洹1，王彦平2，颜亮2，徐浩3，弓健3，刘革修忪 暨南大学医学院’血液病研究所，2病理生理教研室(广州510632)；3暨南大学附属第一医院核医学科(广州 510630) 【摘要】 目的研究小鼠胎盘源干细胞抗臭氧致衰老的功效。方法吸入臭氧法制造，卜鼠衰老模型；剖宫产 取小鼠胎盘，密度梯度离心法分离、腹腔注射法移植小鼠胎盘源干细胞；Y染色体原住杂交法检测细胞植入情况；超 声检查小鼠心脏；各器官做组织学衰老程度比较、评分。结果 移植30d后治疗组小鼠心脏、肾脏、肺脏、皮肤等器 官各衰老指标与模型组之间差异无统计学意叉。结论单纯移植小鼠胎盘源干细胞不能有效地抗臭氧致衰老。 【关键词】 衰老；臭氧；干细胞 The ofmice OilmiceozoBe—inducedmodeluJun。．ZHANG effect stemcell aging placenta transplantation C,e—xiu．。Hematonos／sinstitute Huan，WANGYan—ping，YANLiang，XUHao，GONGJian，／2U ofMedicalSchool，五- llan University，Guangzhou510632，China Ge—xiu Correspondingauthor：LIU Toevaluatetheeffectofmice stemcell onmiceozone model。 【AbstractlObjective placenta transplantation aging via MethodsAmurineoxidativestressmodelwasestablishedviaozoneinhalation．Micewereobtainedcesare珊l placentae section stemcells isolated and into in— were transplatedrecipientsbyperitoneal delivery．Placenta bygradientcentrifugation situYchromosomal was fordetectionofthe and constructed cells．Histologicalscoring jection．In hybridization transplanted of wereassessedfor Therewasno statisticaldifferenceinthese‘ senilitydegreeorgans significantly comparison．Results nileindexof andskinbetweenthetreatmentandthecontrol 30 after heart，kidney，lung group groupdays transplantation． Conclusi