车前对铝胁迫生理响应的研究-热带亚热带植物学报.PDF

热带亚热带植物学报　2014 ，22(5) ：495 ~ 501 Journal of Tropical and Subtropical Botany 车前对铝胁迫生理响应的研究 1 2 3* 胡雪华, 李蕴, 邹天才 (1. 井冈山大学生命科学学院，江西 吉安 343009； 2. 南阳市一中，河南 南阳 473000；3. 贵州科学院，贵阳 550001) 摘要： 为探讨车前(Plantago asiatica)对铝胁迫 的耐受特性及生理机理 ，在不 同铝浓度及胁迫时间下 ，对其 叶片的渗透调节物 质 、膜脂过氧化程度和体 内保护酶系统进行 了研究 。结果表明，低浓度铝处理对车前 的生理指标无 明显影响。随着铝浓度 的升 高 ，叶片脯氨酸、可溶性糖 、可溶性蛋 白质含量呈先升高后下降趋势 ，细胞质膜透性显著增大、MDA 含量显著增加 。500 mg L– 1 的 Al3+ 处理 ，车前 叶片的 SOD 、CAT、POD 活性均 明显提高。因此 ，在铝胁迫下 ，野生草本植物车前能通过体 内的生理保护 机制来减少 Al 胁迫 ，表现出较强的耐铝特性 。 关键词： 铝胁迫 ；渗透调节物质 ；膜脂过氧化 ；保护酶 ；车前 doi: 10.3969/j .issn.1005–3395.2014.05.011 Studies on Physiological Responses to Aluminum Stress of Plantago asiatica 1 2 3* HU Xue-hua , LI Yun , ZOU Tian-cai (1. School of Life Sciences, Jinggangshan University, Ji’an 343009, China; 2. Nanyang No. 1 High School, Nanyang 47300, China; 3. Guizhou Academy of Sciences, Guiyang 550001, China) Abstract: In order to understand the physiological responses and mechanisms to aluminum stress in wild herb Plantago asiatica, the characteristics of osmotica, membranous peroxidation and protective enzyme system under aluminum stress were studied. The results showed that there were no significant effects on physiological indexes of P. asiatica treated with low concentration of aluminum. With increasing of aluminum concentration, the contents of proline, soluble sugar and soluble protein in leaves rose at first and then descended. Under high concentration of aluminum stress, the membrane permeability and MDA content of leaves increased significantly. When treated with 500 mg L–1 Al3+ or above, the activities of SOD, CAT, POD in P. asiatica leaves increased significantly. Therefore, wild herb Plantago asiatica could start physiological protection mechanism to reduce harm by aluminum stress, showing strong resistance to aluminum. Key words: Al stress; Osmolyte; Membrane lipid peroxidation; Protective enzyme; Plantago a