口蹄疫病毒外源序列插入位点的研究进展.pdfVIP

生 物 工 程 学 报 张岩 等/ 口蹄疫病毒外源序列插入位点的研究进展 175 Chinese Journal of Biotechnology /cjbcn February 25, 2014, 30(2): 175−181 DOI: 10.13345/j.cjb.130225 ©2014 Chin J Biotech, All rights reserved 综 述 1 1 2 2 张岩 ，胡永浩 ，杨帆 ，郑海学 1 730070 2 730046 , , , . . , 2014, 30(2): 175−181. ZhangY, Hu YH, Yang F, et al. Progress in insertion sites for foreign sequence of foot and mouth disease virus. Chin J Biotech, 2014, 30(2): 175−181. 摘 要: 伴随着对口蹄疫病毒 (FMDV) 蛋白结构功能的深入研究，发现FMDV 能够表达外源基因片段。通过 对 FMDV 的基因进行改造和修饰研究，进而实现了不同应用目的，如提高病毒滴度、引入标记、提高免疫应 答、降低致病性等。文中主要从FMDV 接受外源基因插入位点角度来介绍现阶段关于FMDV 表达外源基因相 关进展。 : 口蹄疫病毒，外源基因，插入位点 Progress in insertion sites for foreign sequence of foot and mouth disease virus 1 1 2 2 Yan Zhang , Yonghao Hu , Fan Yang , and Haixue Zheng 1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China 2 National Foot and Mouth Disease Reference Laboratory, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China Abstract: With the progess in studying gene structure and function of foot and mouth disease virus (FMDV), FMDV can Received: May 2, 2013; Accepted: July 16, 2013 Supported by: National High Technology Research and Development Program of China (863 Program) (No. 2011AA10A211-1), the High-level Technological Talent Program of Gansu Province (No. 1013JHTA008), the International Atomic Energy Agency (No. 16025/R0), China