地西他滨联合丙戊酸钠对白血病细胞株的促分化效应研究.pdfVIP

地西他滨联合丙戊酸钠对白血病细胞株的促分化效应研究 1 2 刘波 ，邬露丹 (1.宁波市第九医院，浙江 宁波 315020 ；2.宁波市医疗中心李惠利医院，浙江 宁波 315040) 摘要：目的 探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株HL-60 的促分化影响。方 法 设立分组如下：对照组，DCA A 组(1.0 mol·L1) ，DCA B 组(4.0 mol·L1) ，VPA 组(2.0 mmol·L1) ，联合用药A 组(DCA 1.0 mol·L1+VPA 2.0 mmol·L1) ，联合用药B 组(DCA 4.0 mol·L1+VPA 2.0 mmol·L1) ，作用48 h 。流式细胞术检测CD34、 CD117 MFI 以及CD11b、CD14 表达率。结果 联合用药A 、B 组的CD117 和CD34 MFI 显著低于其各自的单药组(P0.01) ； 联合用药 A 组的 CD11b 和 CD14 表达率和联合用药 B 组 CD11b 表达率显著低于其各自的单药组(P0.01) 。结论 VPA 与DCA 联用对HL60 细胞具促分化作用。 关键词：分化抗原；HL-60 细胞；丙戊酸钠；地西他滨 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2012)06-0486-04 Synergistic Effect of Decitabine and Valproic Acid on Induction of Differentiation in Leukemic HL-60 Cells 1 2 LIU Bo , WU Ludan (1.Ningbo No.9 Hospital, Ningbo 315020, China; 2.Ninbo Medical Treatment Center Lihuili Hospital, Ningbo 315040, China) ABSTRACT: OBJECTIVE To investigate the synergistic effect of decitabine(DCA) and valproic acid(VPA) in differentiation induction in leukemic HL60 cells. METHODS The drug groups were set as follows: control group; DCA group A(1.0 μmol·L1); DCA group B(4.0 μmol·L1); VPA group(2.0 mmol·L1); combination group A(DCA 1.0 μmol·L1+VPA 2.0 mmol·L1); combination group B(DCA 4.0 μmol·L1+VPA 2.0 mmol·L1). The cells were treated for 48 h. The CD117, CD34 mean fluorescence intensity(MFI) and CD14, CD11b expression were detected by flow cytometry. RESULTS The CD117, CD34 MFI of combination group A and B were significantly lower than their corresponding concentration single drug group(P0.01). Except CD14 of combination group B, the CD14, CD11b expressions of combination group A and B were signific