基础研究·酒精性骨质疏松症造模鼠的骨与生化代谢.PDF

中国骨伤 2006 年 1 月第 19 卷第 1 期 　China J Ort hop Trauma ,J an . 2006 ,Vol . 19 ,No . 1 ·3 1 · ·基础研究 · 酒精性骨质疏松症造模 鼠的骨与生化代谢 特征 1 2 齐振熙 ,王明千 ( 1 福建中医学院科研处 ,福建 　福州 　350 108 ;2 福建中医学院骨伤系) 　　【摘要】　目的:观察酒精中毒引发骨质疏松造模鼠的骨与生化代谢变化特征 ,探讨酒精对骨质疏松 症形成过程的毒性作用 。方法 :SD 大鼠 120 只分为 3 组 ,实验组 A 给予 45°白酒 8 ml ·kg - 1 ·d - 1 ,每天 2 次灌胃,实验组 B 给予 45°白酒 6 ml ·kg - 1 ·d - 1 ,每天 2 次灌 胃,对照组以 8 ml 生理盐水灌 胃,分别于 8 、16 、24 周取材 ,进行相关骨代谢指标的测定 。结果 :各实验组动物骨小梁稀疏 ,髓腔脂肪细胞增大 、增 多 ;骨细胞数量减少 ,退变固缩或出现脂滴 ,成骨细胞减少 ,尤其实验组 A 改变最为明显 。16 周后实验 组 A 的BMD 显著下降;24 周后骨 Ca 、HOP 含量及 Ca/ HOP 比值均呈显著降低 ;血清 A KP 、B GP 、血 Ca 及钙磷乘积显著下降;而尿 Ca 、P 、HOP 排泄量则呈显著增多 。结论 :酒精中毒可引起模鼠骨代谢紊乱 , 继发骨质疏松 。 【关键词】　酒精中毒 ; 　骨质疏松 ; 　骨代谢 Feature of bone and biochemical metabolism of osteoporotic rat induced by alcohol 　QI Zhenx i , WA N G M ingqian . S cientif ic R esearch I ns tit ute , Traditional Chinese M edicine College of Fuj ian , Fuz hou 350 108 , Fuj ian , China ABSTRACT 　Objective : To observe t he feature of bone and biochemical metabolism of osteoporotic rat induced by alcohol ,and investigate t he effect of alcohol during t he develop ment of osteoporosis. Methods : 120 SpragueDawley rat s were divided into t hree group s randomly ,exp eriment group A was administed 45°distil late spirit 8 ml p er kilogram every day and two times a day ;exp eriment group B was administed 45°distillate spirit 6 ml p er kilogram every day and two times a day ;control group was administed equivalent saline . Sp eci mens were dislodged at t he time of 8 ,16 ,24 weeks and t he index of associated bone metabolism were assayed . Results :The bone trabecula became rarefaction in exp eriment group s ,adipocyte in medullary c