两株溶藻细菌对棕囊藻溶藻作用的原子力显微镜研究.pdfVIP

第29卷第1期 暨南大学学报(自然科学版) Vo1．29 No．1 2008年2月 Journal of Jinan University(Namr~Science) Feb． 2008 两株溶藻细菌对棕囊藻溶藻作用的原子力显微镜研究 杨晓新， 尹平河 (暨南大学水生生物研究所，广东广州510632) [摘 要] 细菌在赤潮生消过程中起着重要的作用．课题组从珠海赤潮发生海域采集的11株细菌中分离、筛选 出2株具有显著溶藻效果的细菌．用原子力显微镜研究结果表明，2株细菌均属杆菌，形态明显不同，均可作用于 球形棕囊藻，导致藻细胞破裂和胞内物质溶出而死亡，达到治理棕囊藻赤潮的目的． [关键词] 溶藻细菌；溶藻作用； 棕囊藻；原子力显微镜 [中图分类号] Q949．2；X55 [文献标识码] A [文章编号] 1000—9965(2008)01—009o一05 Study on the two of algae-lysing bacteria which control red-tide of Phaeocystis globosa by AFM YANG Xiao—xin， YIN Ping—he (Institute of Hydrobiology，Jinan University，Guangzhou 510632，China) [Abstract] Algae—lysing bacteria is a kind of sea bacteria accompanied with red tide，which can inhib— it or kill algae cel1．Eleven bacteria were separated from red tide zone in Pearl Sea，from which 2 with re— markable algae—lysing effect were isolated．Their characters and inhibition on growth of Phaeocystis globo— sa were studied by AFM．The results showed that both of them were bacillus but with different configura— tion．Both could inhibit P．globosa by breaking algae cell surface layer and making the inclusion of algae cell released until P．globosa was lysed and died finally，which provided both theory and practice method f0r contolling the red tide of P．globosa． [Key words] algae—bacteria； algae—lysing；Phaeocystis globosa；AFM 目前，有害赤潮已经成为全球性的海洋灾害． 制藻类生长，杀死藻细胞，从而消灭有害赤潮．溶藻 仅1997—2006的10年时间，中国海域发生的赤潮 细菌作为赤潮防治的新方法和可能微生物，开始引 面积累计已达95 000平方公里，直接经济损失近50 起广泛关注． 多亿元．鉴于赤潮在各方面造成的严重影响，研究 藻类与细菌有着密切的关系．近几年文献报道 赤潮防治方法、制定有关防治对策已经成为许多国 了一些溶藻的细菌菌株H ．探究藻菌关系和溶藻 家和地区的当务之急．赤潮治理方法主要有物理方 细菌对水体进行生态修复是利用微生物方法治理有