酶解破壁促进废次烟叶中茄尼醇溶浸.PDFVIP

1706 王星敏 等/酶解破壁促进废次烟叶中茄尼醇溶浸 生 物 工 程 学 报 Chinese Journal of Biotechnology November 25, 2013, 29(11): 1706−1710 /cjbcn ©2013 Chin J Biotech, All rights reserved 生物育种与工艺优化 酶解破壁促进废次烟叶中茄尼醇溶浸 王星敏，张渝文，张桂芝，殷钟意 重庆工商大学环境与生物工程学院，重庆 400067 王星敏, 张渝文, 张桂芝, 等. 酶解破壁促进废次烟叶中茄尼醇溶浸. 生物工程学报, 2013, 29(11): 1706−1710. Wang XM, Zhang YW, Zhang GZ, et al. Improved extraction of solanesol from tobacco waste by enzymatic cell wall breaking. Chin J Biotech, 2013, 29(11): 1706−1710. 摘 要: 协同应用纤维素酶和木质素酶催化降解废次烟叶，探讨清洁高效的酶解破壁效应及浸提茄尼醇工艺 条件。结果发现复配酶催化裂解溶浸茄尼醇效果明显优于单一酶，酶解时间、温度、pH 值以及酶投加量等条 件均影响酶破壁浸提茄尼醇能效。结果表明，采用纤维素酶：木质素酶酶活比 15 ∶1 (U/U) 的复配酶，在体积 为5 倍烟草质量的水介质环境中，当复配酶投加量为 175 U/g，水浴温度40 ℃，pH 为6 时，催化酶解烟叶8 h 后，茄尼醇溶浸浓度可达0.33 g/L 。在此条件下，茄尼醇平均提取率可达96.53 ％，是化学回流浸提方法的1.68 倍。该方法为有效提取废次烟草中茄尼醇提供了一种新途径。 关键词: 酶促反应，茄尼醇，废次烟草，破壁，提取 Improved extraction of solanesol from tobacco waste by enzymatic cell wall breaking Xingmin Wang, Yuwen Zhang, Guizhi Zhang, and Zhongyi Yin College of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China Abstract: To improve the extraction of solanesol from tobacco waste, we developed an enzymatic cell wall-breaking process with combined cellulase and ligninase. The effects of reaction time, temperature, pH and enzyme/substrate ratio were determined. The results show that the catalytic effect was better than either single enzyme when the ratio of cellulase to ligninase was 15:1 (U/U). Under the opti