青心酮防治ApoE（--)小鼠主动脉粥样硬化斑块形成与脂氧.PDFVIP

青心酮防治ApoE(-/-)小鼠主动脉粥样硬化斑块形成与脂氧素的关系 * 陈鲁曼，张代娟 ，王婷，王琳(潍坊医学院病理生理教研室，山东 潍坊 261053) 摘要：目的 观察青心酮防治ApoE(-/-) 小鼠主动脉粥样硬化(AS)病变形成与脂氧素的关系。方法 取8 只8 周龄C57BL/6 小鼠作空白对照组；取24 只8 周龄的ApoE(-/-) 小鼠，♂，随机分成3 组(每组8 只) ：动脉硬化组(乙醇 20 mg kg1d1) ； 青心酮组(青心酮 20 mgkg1d1) ；辛伐他汀组(辛伐他汀 20 mgkg1d1) 。所有小鼠均以“西方类型膳食”饲养至16 周。 取血检测脂氧素、血脂和 15-脂氧合酶蛋白的含量等；取主动脉根部行HE 染色观察ApoE(-/-)主动脉粥样硬化病变情况； 电镜观察斑块内皮细胞变化。Western blotting 法测定血清中15-LO 水平。结果 青心酮组脂氧素增加，血脂含量降低， AS 病灶形成减少，斑块内皮细胞损伤减轻，15-脂氧合酶含量减少。结论 青心酮防治ApoE(-/-) 小鼠AS 病变，可能与 其增加 15-脂氧合酶途径产物——脂氧素有关。 关键词：青心酮；脂氧素；15-脂氧合酶；动脉粥样硬化 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2012)03-0195-04 Relationship between 3,4-Dihydroxyaceto-phenone Prevention of Atherosclerosis and Lipoxin in ApoE(-/-) mice * CHEN Luman, ZHANG Daijuan , WANG Ting, WANG Lin(Department of Pathophysiology, Weifang Medical College, Weifang 261053, China) ABSTRACT: OBJECTIVE To observe the relationship between 3,4-Dihydroxyaceto-phenone(DHAP) prevention of atherosclerosis(AS) and lipoxins(LXs) in ApoE(-/-) mice. METHODS There were 8 eight-week-old C57BL/6 mice in the normal control group; 24 eight-week-old male ApoE (-/-) mice were randomly divided into three groups: AS group (n=8, ip. alcohol 20 mg·kg1·d1); DHAP treatment group (n=8, ip. DHAP 20 mg·kg1·d1); simvastatin treatment group (n=8, ip. simvastatin 20 mg·kg1·d1). All mice were fed with a Western diet (21% fat, 0.15% cholesterol) for 16 weeks. Their blood was collected for determination of blood lipids and lipoxin concentration. Sections of aortic root were stained by HE. The structures of cells were observed by electron microscope. Western-blotting was applied to detect 15-LO protein in serum. RESULTS The concentration of LXs increased in DHAP-treated group. The concentration of TG and TC decreased in DHAP-treated group; the