银线莲药材HPLC指纹图谱研究.PDFVIP

银线莲药材 HPLC 指纹图谱研究 黄丽萍1 2* 3 2 ，黄丽英 ，李喜载 ，俞晓玲 (1.福建医科大学附属第一医院，福州 350005 ；2.福建医科大学药学院，福州 350004 ； 3.福建农林大学食品科学学院，福州 350002) 摘要：目的 建立银线莲的 HPLC 指纹图谱分析方法，为规范银线莲药材的质量标准提供基础研究资料。方法 采用 − 1 − 1 Agilent TC-C 18 色谱柱(4.6 mm×250 mm ，5 μm) ，流动相为甲醇-0.02 mol·L 乙酸铵溶液，梯度洗脱，流速：1.0 mL·min ， 检测波长：260 nm ，柱温：30 ℃。结果 10 批银线莲指纹图谱的相似度均0.90 ，确定了20 个共有峰，指认了4 个特征 峰，建立了该药材的高效液相色谱指纹图谱。结论 该指纹图谱方法准确可靠，重复性好，能很好地区分开金线莲、银 线莲，可作为银线莲药材内在质量评价的依据。 关键词：银线莲；高效液相色谱法；指纹图谱 中图分类号：R284.1 文献标志码：B 文章编号：1007-7693(2015)10-1170-05 DOI: 10.13748/ki.issn1007-7693.2015.10.003 Fingerprint Chromatography of Goodyera matsumurana by HPLC 1 2* 3 2 HUANG Liping , HUANG Liying , LI Xizai , YU Xiaolin (1.The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China; 2.College of Pharmacy, Fujian Medical University, Fuzhou 350004, China; 3.College of Sitiology, Fujian Agriculture and Forestry University, Fuzhou 350002, China) ABSTRACT: OBJECTIVE To establish fingerprint chromatography of Goodyera matsumurana by HPLC and provide a basic research data for the quality standard of Goodyera matsumurana. METHODS Agilent TC-C 18 column(4.6 mm×250 mm, 5 μm) was adopted. The mobile phase was methanol-0.02 mol·L− 1 ammonium acetate with gradient elution at the flow rate of − 1 1.0 mL·min . The detection wave length was 260 nm. The column temperature was 30 ℃. RESULTS The similarity of 10 batches of Goodyera matsumurana was 0.90. Twenty common peaks were indicated. Four peaks were identified. The fingerprint chromatography of Goodyera matsumurana w