应用酵母双杂交体系筛选FXR1P相互影响蛋白.pdf

目 录 主要英汉缩略语名词对照表„„„„„„„„„„„„„„2 中文摘要„„„„„„„„„„„„„„„„„„„„„„3 英文摘要„„„„„„„„„„„„„„„„„„„„„„4 前言„„„„„„„„„„„„„„„„„„„„„„„„6 一、 材料和方法„„„„„„„„„„„„„„„„„„9 1、 材料„„„„„„„„„„„„„„„„„„„„„9 2 、 方法„„„„„„„„„„„„„„„„„„„„„12 二、 实验结果„„„„„„„„„„„„„„„„„„„20 三、 讨论„„„„„„„„„„„„„„„„„„„„„26 四、 结论„„„„„„„„„„„„„„„„„„„„„30 参考文献„„„„„„„„„„„„„„„„„„„„„„31 综述„„„„„„„„„„„„„„„„„„„„„„„„34 附录„„„„„„„„„„„„„„„„„„„„„„„„42 攻读硕士期间发表的论文„„„„„„„„„„„„„„„45 致谢„„„„„„„„„„„„„„„„„„„„„„„„46 1 主要英汉缩略语名词对照表 缩略语 英 文 中 文 AD activation domain 激活域 BD binding domain 结合域 CDS coding sequence 编码区序列 UAS Upstream activating sequence 上游顺式激活序列 DMF Dimethylformadie 二甲基甲酰胺 DMSO Dimethyl sulfoxide 二甲基亚砜 DO dropout supplements 省却成分添加剂 Ade Adenine 腺嘌呤 His Histidine 组氨酸 Trp Tryptophan 色氨酸 Leu Leucine 亮氨酸 PEG Polyethylene glycol 聚乙二醇 YNB yeast nitrogen base without amino 无氨基酸的酵母氮源 acids SD-X SD media lacking X “X”成分缺陷型SD X-DO X-DO Supplement “X”成分缺陷型添加剂 X -Gal 5-bromo-4-chloro-3-indolyl- D- 5-溴-4-氯-3-吲哚-D-左旋半乳 Galactoside 糖苷 FXR1P/2P fragile X mental retardation-related 脆性X 智力低下相关蛋白1/2 protein 1/2 FXR1/2 fragile X mental retardation, 脆性X 相关基因1/2 autosomal homolog 1/2 FMR1 fragile X mental retardation 1 脆性X 智力低下基因 SAFB scaffold attachment factor B 骨架黏附因子B 17β-HSD Ⅰ Estradiol 17 beta-dehydrogenase 1 17β-类固醇脱氢酶1 B