荔枝果肉乙酸乙酯粗提物对Th1Th2细胞平衡的影响.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.7 荔枝果肉乙酸乙酯粗提物对Th1/Th2 细胞 平衡的影响 杨瑞丽，唐晓恩，周杨，吴青，孙远明 （广东省食品质量安全重点实验室华南农业大学食品学院，广东广州 510642） 摘要：本研究分析荔枝果肉乙酸乙酯部粗提物对小鼠细胞因子分泌的作用，进而探讨其对Th1/Th2 细胞平衡的影响。将 30 只 BALB/C 小鼠随机分成对照组（生理盐水）、乙酸乙酯部粗提物低剂量和高剂量组（4.6、9.2 mg/kg·bw ·d），连续灌胃7 d，观察各组 小鼠的胸腺和脾脏指数变化以及脾细胞增殖情况，用ELISA 检测小鼠脾细胞以及血清分泌IFN-γ 和IL-4 的含量以及IFN-γ/IL-4 比值。 实验结果表明：乙酸乙酯部粗提物高剂量组小鼠的胸腺指数、脾脏指数显著提高。乙酸乙酯部粗提物低、高剂量都能显著提高小鼠脾 细胞和血清IFN-γ 分泌水平，同时，高剂量组小鼠脾细胞和血清IL-4 的分泌水平也显著提高。低、高剂量的乙酸乙酯部粗提物都能 显著提高小鼠脾细胞和血清IFN-γ/IL-4 的比值。荔枝果肉乙酸乙酯部粗提物诱导Thl/Th2 平衡向Th1 方向漂移，可能进而诱导机体的 炎症性反应。 关键词：荔枝果肉；乙酸乙酯部粗提物；Th1/Th2 细胞平衡 文章篇号：1673-9078(2013)7-1467-1470 Effect of Ethyl Acetate Extracts from Litchi Fruit on the Th1/Th2 Cell Balance in Mice YANG Rui-li, TANG Xiao-en, ZHOU Yang, WU Qing, SUN Yuan-ming (Key Laboratory of Food Quality and Safety of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou 510642, China) Abstract: The effect of ethyl acetate extracts (EAE) from litchi fruit on cytokine production of mice was analyzed, and then its effect on the Th1/Th2 cells balance was explored. 30 BALB/C mice were divided into the control group (NC), the low dose group (EL, 4.6mg EAE/kg·bw ·d), and the high dose group (EH, 9.2mg EAE/kg·bw ·d). After 7 d, the index of spleen and thymus gland, and the spleen cells proliferation were detected. The secretion of IFN-γ and IL-4 in spleen cells and serum were determined by ELISA. The results indicated that high-dose EAE could increase the index of spleen and thymus gland. Compared with NC group, IFN-γ secretion IL-4 of spleen cells and serum in EL and EH groups were significantly increased. Higher secreti