2011 惊厥 食品科学 杜仲雄花水溶性生物碱的镇静催眠作用.pdfVIP

296 2011, Vol. 32, No. 11 食品科学 ※营养卫生 杜仲雄花水溶性生物碱的镇静催眠作用 李 欣，刘 严，朱文学，郭 菡，刘少阳 (河南科技大学食品与生物工程学院，河南洛阳 47 1003) 摘 要：目的：研究不同剂量杜仲雄花水溶性生物碱的镇静催眠作用。方法：采用酸水提取法提取杜仲雄花中水 溶性生物碱。观察不同剂量杜仲雄花水溶性生物碱对小鼠自主活动的影响以及直接镇静催眠作用、与戊巴比妥钠的 协同作用和抗惊厥作用。结果：杜仲雄花水溶性生物碱能有效减少小鼠的自主活动次数；与戊巴比妥钠有较好的 协同作用，能显著增加阈下剂量的小鼠睡眠率，延长阈下剂量及阈上剂量的睡眠时间，并缩短睡眠潜伏期；杜仲 雄花水溶性生物碱还能有效降低尼可刹米所致的小鼠惊厥率，延长惊厥潜伏期。结论：杜仲雄花水溶性生物碱具 有良好的镇静催眠作用。 关键词：杜仲雄花；水溶性生物碱；镇静；催眠 Sedative and Hypnotic Effects of Water-soluble Alkaloids from Male Flowers of Eucommia LI X in ，LIU Y an ，ZHU Wen -xu e ，GUO Han ，LIU Shao-yan g (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 47 1003, China) Abstract ：Objective: To explore the sedative and hypnotic effects of different doses of water-soluble alkaloids from male flowers of Eucommia. Methods: Water-soluble alkaloids were extracted from Eucommia male flowers by an acidic water extraction method, their effect on spontaneou s activities of mice, hypnotic function with and without the presence of pentobarbital sodium and anticonvulsant effects were investigated at different doses. Results: Water-soluble alkaloids from Eucommia male flowers could decrease the frequency of spontaneous activities, significantly increase sleeping ratio below the threshold dose, prolong the sleeping time and shorten the sleeping latency. Meanwhile, these alkaloids also could effectively reduce the eclamptic ratio of the mice and significantly prolong the convulsion latency. Conclu sion : Water-soluble alkaloids of E ucommia male flowers have obviou sly sedative and hypnotic effects in mice and there is a synergistic effect between them and pentobarbital sodium. Key words：E ucomm ia m ale flow er s ；water-soluble alkaloids ；sedation ；hypn osis 中图分类