纳米金探针在基因检测中的应用 - 生命科学.pdf

第21卷 第3期 生命科学 Vol. 21，No. 3 2009年6月 Chinese Bulletin of Life Sciences Jun.，2009 文章编号 ：1004-0374(2009)03-0461-06 纳米金探针在基因检测中的应用 周　政 (华南理工大学广州汽车学院物理教研组，广州 510800) 摘　要：传统的核酸分析中常采用放射性元素、荧光色素以及酶标记等基因探针，这些探针都存在着 一些不足之处。 近年来，纳米金探针作为一种新型的基因探针，已引起了广泛的关注。该探针具有 优良的光谱特征和光化学稳定性，对核酸的非特异吸附性小，与核酸等生物大分子结合后不改变生物分 子的活性。将纳米金探针用于基因检测，具有操作简便、快速、安全、实验成本低等优点。本文 就纳米金探针的发展过程、纳米金探针的制备、检测原理及其在基因分析中的应用等几个方面作了系统 而全面地概述，同时介绍了纳米金探针的最新研究进展，并对其发展前景作了简要评述。 关键词：纳米金探针；基因检测；检测模式分析 中图分类号：Q67; Q781　　文献标识码：A The application of gold nanoparticles probe in gene detection 　 ZHOU Zheng (Teaching and Research Group of Physics, College of Guangzhou Auto, South China University of Technology, Guangzhou 510800, China) Abstract: Traditional methods for gene analysis often use the radioactive, the fluorescent, or the enzymatic probes. These probes all have some disadvantages. Recently, gold nanoparticles as a new kind of gene probe have attracted wide attention. It has excellent spectral character and photochemical stability, and has little nonspecific absorption to the nucleic acids. The bioactivity of biomacromolecule wouldn’t be changed after absorption on gold nanoparticles. The application of gold nanoparticles probe in gene detection has many advantages, such as simple, rapid, safe and inexpensive. This paper introduced the preparation of the gold nanoparticles probe, the detection principle, the application and the latest research progress of the probe. Key words: gold nanoparticles probe; gene detection; detection mode analysis 随着人类基