脑外伤后抑郁状态模型建立及效果评价.docVIP

脑外伤后抑郁状态模型建立及效果评价【摘要】目的 探讨建立脑外伤后抑郁状态动物模型，并对此模型的建立和有效性做系统评价。方法 本实验在武汉大学医学院实验中心完成。选用48只雄性Wistar大鼠，随机(随机数字法)分为4组，每组12只。①假手术组：每笼6只饲养，正常饮食饮水；②模型组：以自由落体撞击法制作大鼠左侧额叶脑挫裂伤模型，每笼1只饲养，术后1周加以慢性应激和孤养方法，造成脑外伤后抑郁状态动物模型；③脑外伤组：以自由落体撞击法制作大鼠左侧额叶脑挫裂伤模型，每笼6只饲养，正常饮食饮水；④慢性不可预见性温和应激组：每笼1只饲养，采用慢性应激方法结合孤养法造成大鼠慢性应激抑郁模型。 观察各组大鼠蔗糖水试验，自发性行为改变，用高效液相色谱荧光检测法测定各组大鼠脑内海马、前额叶、下丘脑和纹状体不同部位的多巴胺、5-羟色胺、 去甲肾上腺素单胺类神经递质含量。采用SPSS 13.0统计软件分析结果，资料采用单因素方差分析或成组t检验，以P 0.05为差异具有统计学意义。结果 造模后即予行为学测试，模型组大鼠蔗糖水饮用量逐渐减少，反映模型组大鼠兴趣和快感缺乏；旷野试验中模型组大鼠水平及垂直得分减少，反映模型组大鼠活动性减少，兴趣缺乏；模型组大鼠游到终点的时间延长，错误次数增加，反映模型组大鼠的学习和记忆能力下降；模型组大鼠海马、前额叶、下丘脑，和纹状体多巴胺、5-羟色胺、去甲肾上腺素含量下降。结论 在左侧额叶脑挫裂伤模型的基础上给予孤养和慢性不可预见的温和性应激，能建立较理想的脑外伤后抑郁状态动物模型。 【关键词】脑外伤；抑郁；动物模型;单胺类神经递质;形态学 Establishment and evaluation of model of depression after traumatic brain injury CHU Sheng-hua, CHEN Er-tao, FENG Dong-fu, MA Yan-bin, ZHU Zhi-an. Departmet of Neurosurgery, NO.省略 【Abstract】Objective To discuss about the establishment of mental depression model by traumatic brain injury in rats， and to evaluate the validity of this model. Methods The study was carried out in the experiment center of Wuhan University. A total of 48 male adult Wistar rats were randomized（random number） into four groups (n=12, each): (1) in sham operation group, six rats were fed normally in one cage; (2) in model group, the model of the left frontal lobe contusion was made up by hitting with free-fall method on the left cortex of the rat, and subsequently the each of model rats were separately fed in a cage and put them in a lonely environment with chronic stress one week after traumatic brain injury in order to induce them into models of mental depression; (3) in brain trauma group, the trauma model of the left frontal lobe contusion was set up by the same procedure as in model group, and six rats were fed together in one cage; (4) in CUMS group, each rat was fed normally in one cage in a lonely environment after brain trauma made and chronic mild chaotic stress unpredictable to rats was given to induce me