医用TH胶栓塞建立月骨缺血性坏死兔模型整理.docVIP

医用TH胶栓塞建立月骨缺血性坏死兔模型 路云翔，陈郁鲜，庄? 泽，任建华，彭? 优，史德海，王? 昆，李智勇 中山大学附属第三医院关节外科、创伤骨科，广东省广州市?? 510630 Medical TH adhesive embolism for establishing a rabbit model of ischemic necrosis of lunate bone Lu Yun-xiang, Chen Yu-xian, Zhuang Ze, Ren Jian-hua, Peng You, Shi De-hai, Wang Kun, Li Zhi-yong Department of Joint Surgery and Orthopedic Trauma, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China 背景：目前缺乏与人类月骨缺血性坏死病理过程类似的Kienb?ck病动物模型。 目的：采用医用TH胶栓塞法建立一种新型月骨缺血坏死兔模型，并探讨造模方法的合理性。 方法：选择健康成年新西兰兔30只，雌雄不限。采用自身对照方法随机分为实验侧和对照侧，实验侧采用月骨中心钻孔，注射医用TH胶0.2 mL，反复注药3次；对照侧月骨中心钻孔注射同等量的生理盐水。于实验4，8，12周行X射线、大体观察、Micro-CT 骨计量学及组织病理学检测。 结果与结论：大体标本、X射线及组织学检测结果可见实验侧造模后8周月骨出现缺血坏死表现，12周月骨缺血坏死更为典型。Micro-CT 骨小梁微观参数中，实验侧骨小梁密度参数骨体积分数及骨小梁数量均明显低于对照侧(P? 0.05)；骨小梁空间参数明显增高，其中实验侧骨小梁离度及结构模型指数明显高于对照侧。提示注射医用TH胶栓塞后8周实验侧出现月骨缺血坏死表现，注药后12周可建立月骨缺血坏死兔模型，可以实现在周围组织无明显损伤情况下出现月骨内血运受损、骨小梁骨折、空骨陷窝等与人类月骨坏死类似的改变。 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程 全文链接： 关键词?：?实验动物；?组织构建；?Kienb?ck病；?月骨；?动物模型；?TH胶；?骨小梁；?Micro-CT；?X射线； ?? Abstract： BACKGROUND:?Kienb?ck disease lacks of suitable animal models, which are similar to the pathological process of avascular necrosis of human lunate bone. OBJECTIVE:?To establish a new animal model of Kienb?ck disease using medical TH adhesive embolism and to explore the rationality of model establishment. METHODS:A total of 30 healthy adult New Zealand rabbits, male or female, were selected. Using self-control method, the rabbits were randomly assigned to experimental sides and control sides. By drilling in the center of the lunate bone, 0.2 mL of medical TH glue was injected three times. An equal volume of physiological saline was injected into the center of the lunate bone on the control side. X-ray examination, general observation, Micro-CT measurement of bone, and tissue pathology detection were conducted at 4, 8 and 12 weeks. RESULTS AND CONCLUSION:Gross specimen, X-ray and histological results showed that ischemic necro