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ms培养基的配制与灭菌(Preparation and sterilization of MS medium)
ms培养基的配制与灭菌(Preparation and sterilization of MS medium)
Preparation and sterilization of MS medium
A common medium in plant tissue culture is the MS medium. The preparation of the MS medium comprises the following steps.
Preparation and preservation of MS culture medium containing nearly 30 kinds of nutritional components of base liquor, in order to avoid every medium preparation should be carried out on the weighing dozens of ingredients, can be cultured in the medium of various components, according to the original amount of 20 times or 200 times respectively weighing, with concentrated liquid, this concentrated solution is called culture base liquor. In this way, each use, take the total amount of 1/20 (50 mL) or 1/200 (5 mL), dilute with water, into the culture medium. The quantities of various substances required for the preparation of the mother liquid of the culture medium are listed for use in preparation.
A large number of elements (mother liquor I) mg / L
NH4NO3 33000
KNO3 38000
CaCl2 2H2O 8800
MgSO4 7H2O 7400
KH2PO4 3400
Trace elements (Mother Liquor II)
KI 166
H3BO3 1240
MnSO4 4H2O 4460
ZnSO4 7H2O 1720
Na2MoO4 2H2O 50
CuSO4 5H2O 5
CoCl2 6H2O 5
Iron salt (mother liquor III)
FeSO4 7H2O 5560
Na2-EDTA 2H2O 7460
Organic ingredients (mother liquor IV)
IV A
Inositol 20000
IV B
Niacin 100
Pyridoxine hydrochloride (vitamin B6) 100
Thiamine hydrochloride (vitamin B1) 100
Glycine 400
The amount of all the nutrients mentioned above was 200 times of concentrated liquid except for mother liquor, which was 20 times concentrated liquid.
Each of these mother liquids shall be individually separated into 1 L stock solution. The preparation method of mother liquor, liquor and liquor are: II IV weighing several components each in the mother liquor after respectively with distilled water to dissolve a small amount, and then they are immiscible, the final volume to 1 L.
The preparation method is: Liquor III will be called FeSO4 7H2O and Na2-EDTA 2H2O respectively in 450 mL dis
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