建立不同程度弥漫性轴索损伤动物模型-第三军医大学学报.docVIP

建立不同程度弥漫性轴索损伤动物模型 向玲 张雨婷 梁平 韦红 彭玲珑 李禄生 重庆医科大学附属儿童医院小儿外科（国家重点临床专科）神经外科，重庆，400014 diffuse axonal injury, DAI）装置，建立不同程度DAI动物模型。方法 运用自制的实验装置致伤，使大鼠的头颅同时产生瞬间超大角加速度和线加速度。在恒定气压下，通过不同的旋转往返加速运动次数，造成轻型、中型、重型三种不同程度的大鼠DAI损伤模型，进行行为及病理效应评价。结果 损伤急性期致伤组大鼠的神经生理反射恢复的时间间隔与损伤程度成正相关（P0.05）。致伤7天后致伤组大鼠的神经功能评分与损伤程度成负相关（P0.05）。随着损伤程度的加重，DAI病理特征性改变更为明显。结论 本装置能有效建立不同损伤程度的DAI动物模型，且具良好的稳定性和重复性。 【关键词】弥漫性轴索损伤；实验装置；动物模型；大鼠 Establishment of rat model of different degree diffuse axonal injury XIANG Ling1, ZHANG Yu-ting2 Liang Ping1, WEI Hong3, Peng Ling-long1, Li Lu-sheng1 Department of neurosurgery, Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center For Child Development, Chongqing, 400014, China. 【Abstract】 Objective To design a new experimental facility to induce different degree rat diffuse axonal injury (DAI) model. Methods A total of 70 healthy adult SD rat were randomly divided into normal control group(n=10) and DAI group(n=60), and DAI group was randomly subdivided into group A, B, and C, with 20 rats in each group. Rat head injury was made by the new experimental facility, which was developed to let the rat head span at the moment and ensure a largely combined linear and angular accelerations injury to the head under stable pressure (0.8Kpa). Group A, B, and C were given respectively once, twice, and three times rotating accelerated motion back and forth, which induce light, medium and heavy rat DAI model, respectively. Completing pathological examination and behavior score to verify the model. Results At the acute phase (24 hours, complete a measurement every minute until rats return to normal) , the interval time of nerve reflex recovery in group A, B, and C positively related to the the number of rotating movement (P0.05). The nerve function score in DAI group negatively related to the number of rotatin