梯度密度离心分离磁性纳米颗粒.pdfVIP

Journal of Chromatography A, 1218 (2011) 3823–3829 Contents lists available at ScienceDirect Journal of Chromatography A j o u rn al h omepage: w w w. elsev /locate/chro ma Separation of nanorods by density gradient centrifugation Bin Xiong, Jing Cheng, Yanxia Qiao, Rui Zhou, Yan He ∗ , Edward S. Yeung College of Chemistry and Chemical Engineering, College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China a r t i c l e i n f o a b s t r a c t Article history: The experimental conditions necessary for the synthesis of well-defined nanoparticles are often difficult Received 26 December 2010 to control. There is thus a compelling need for post-synthesis separation of nanoparticles polydispersed Received in revised form 11 April 2011 in size and shape. We demonstrate here both theoretically and experimentally that gold nanorods with Accepted 12 April 2011 diverse aspect ratios can be separated using density gradient centrifugation. By analysing the force bal- Available online 20 April 2011 ance of a Brownian rod falling in a Stokes flow, we derive a rigorous and predictive model that reveals the quantitative dependency of the nanorod sedimentation rates on their mass and shape. The calculations Keywords: show that while mass dependency is still the dominating factor during centrifugation, the shape factor Gold nanoparticle Nanorods