复元活血汤对大鼠骨折转化生长因子β1、血清Ⅰ型胶原、骨钙素表达影响.pdf

Fu Yu an H uo X ue D ecoetion im Pactin g the exPression 0fT G F ·p ;、 C o lla g en l 、 B G P d u rin g in R a ts d u r in g F r a ctu re H ea lin g SP eciality :O rthoP aedics and Tranm atology ofT C M A u th o r :Z h an g H en g l b to r :P ro f.X ie J in A b str a C t O bjective:O bserve the effe etsofFu Yu an H uo X ue (碑 H X )D ecoetion on serum bone ganllna C arboxyglutam ate Protein (B G P)，C ollagen l，肠ansform ing grow th faetor一pl (T G F 一p l)，genetie exPression of eallus of ftaeture sides at level of m R N A and Protein， d u rin g rat freatu re h ea lin g v ia P araffi n seetio n ，x 一ray .M eth o d s :C lo se d tib ia l ftaetu re o f th e leftfem u r w a s in du eed in an esth etiZed 7 0 S P ragu e一D aw ley rats u sin g a stan d ard ized m o d e l for P ro d u etio n o f d isP lay seal fraetu res u sin g a m o d ified teeh n iqu e .F ae ture sid es w ere fixed by Percutaneous intram edullary nails(rats uP to eomm and)，6 norm alrats served as co n tro l gro u P .A l l rats w ere filled w ith F Y H X D eeo etio n served a s treatm en t gro u P w h ile n o nn al so d iu m served as eo n tro l gro u P after fraeture .B lo o d serum w a s d e term in ed b y the expression of serum B G 只 C ollagen 1 b efore ftaeture an d on th e 3 h‘，5 h‘，7 h‘，14 h‘，2 1 h‘day aft er ftaeture (n 6/tim e Point).C allus of fraeture w ere determ ined by the T G F一p lm R N A and protein expression on the 3，h ，5，h，7 h‘，14，h，21h‘day (n 6/tim e point) via reverse transeriPtase Polynl erase chain reaetion (R l’P-C R ) and enzym e link ed im m unosorbent assay (E LISA ).Re su lts :FY H X D eeoetion can Prom ote the exPressions of serum B G P， C o llage n l，T G F 一p 2 of eallu s o f fraetu r