电针对肥胖大鼠胰岛素抵抗及肿瘤坏死因子_的影响_龚美蓉.pdfVIP

辽宁中医杂志 2010年第 37卷第 7期 # 1387# A , , , (南京中医药大学针药结合实验室, 江苏 南京 210029) : 目的:观察针刺对肥胖大鼠体重血糖胰岛素含量脂肪组织中肿瘤坏死因子 - A (TNF- A) 平的影响 方法: 随机挑选 10只普通饲料喂养为正常组另一组 80只高脂致肥饲料喂养,将造模成功的肥胖大鼠 30只随机分为 肥胖模型组和针刺组, 每组各 15只采用实时定量 PCR技术测定肿瘤坏死因子- A (TNF- A)基因表达 平, 酶联免疫 测定法测定血清中胰岛素( insulin)的含量, 生化比色法测定血清葡萄糖含量结果: 针刺治疗组体重血糖血清胰岛素 脂肪组织中TNF- A 平均较模型组有不同程度的降低, 胰岛素敏感指数有所提高结论: 针刺可以改善肥胖大鼠胰岛 素抵抗和糖代谢紊乱状态 : 胰岛素抵抗;肿瘤坏死因子;肥胖 : R245197 : A : 1000- 1719( 2010) 07- 1387- 03 E ffect of E lectroacupuncture on In su lin R esistance and TNF - A in Ob ese rats GONGM ei-rong, XU B in,MAO Zhen, SHAO Q ing-hua (Laboratory of PunctureN ChineseM edicine,Nanjing University of Traditionl ChineseM edicine, Nanjing 210029, Juangsu, China) A bstrac t: Objective: To observe the effect of acupuncture on body weight, blood glucose, serum insulin contents and the ex- pression ofTNF- A in fat tissue in obese rats.M ethods: Randomly selected 10 rats fedw ith ordinarywhole priced foragewere used as normal control group; Another group of 80 rats fedw ith high fat forage. Successfully established expermi entalmodel ratswere random ly divided into two groups: one group without any treatment asmodel control group( control group, n= 15) , and the other treatedw ith acupuncture( acupuncture group, n= 15). Serum insulin contents were measured by Elisa, and TNF- A mRNA was detectedw ith SYBR Green quantitative real- tmi e PCR. The changes of body weight, contents ofblood glucose and serum insulin as well as expression ofTNF- Agene in islets in the obese rats were observed before and after acupuncture. Results: T