半枝莲中的黄酮抗幽门螺杆菌作用研究.doc

半枝莲中的黄酮抗幽门螺杆菌作用研究 肖兰青，熊晓虎 （湖北省武汉市湖北经济学院医院内科，湖北武汉，430205） [摘要] 目的：以活性为导向，从中药半枝莲中分离提取具有抗幽门螺杆菌（Helicobacter pylori）活性的活性成分，并对其抗菌活性进行验证。方法：以活性为导向，采用硅胶、Sephadex LH-20、ODS RP-18反向硅胶等柱色谱方法进行分离纯化，最后通过对照品进行色谱法比对鉴定化合物结构，并通过测定MIC明确其活性。结果：从中药半枝莲中分离得到活性明确的黄酮类化合物3个，分别为：山奈酚-3-O-鼠李糖苷(MIC 15.63 μg/mL)、山奈酚-3-O-葡萄糖苷(MIC 15.63 μg/mL)、山奈酚-3-7-二鼠李糖苷(MIC 31.25 μg/mL)。结论：从半枝莲中分离得到的这三个化合物均具有较好的抗幽门螺杆菌活性，并能降低氨苄西林对幽门螺杆菌的MIC (0.24 0.03 μg/mL)。 [关键词] 半枝莲；幽门螺杆菌；黄酮 Flavonoids with anti-Helicobacter pylori activity from Scutellaria barbata. 作者1，作者2，作者3，通讯作者* (作者单位 作者科室，城市 邮政编码) Abstract objective: We designed this study to isolate and define the active component(s) involved in the anti-Helicobacter pylori activity of the extract of Scutellaria barbata through activity-guided fractionation procedures. Method: The constituents were isolated and purified by repeated column chromatography on silica gel, Sephadex LH-20 and ODS RP-18. The chemical structure of the compounds were elucidated by comparing its high performance liquid chromatography (HPLC) with that of the standard substance, and their anti-H. pylori activity were tested in vitro and was measured as minimum inhibition concentration (MIC). Result: Three compounds with anti-H. pylori activity were isolated and identified as: kaempferol-3-O-α-L-rhamnoside (MIC 15.63 μg/mL), kaempferol-3-O-α-L- glucoside (MIC 15.63 μg/mL), Kaempferitrin (MIC 31.25 μg/mL). Conclusion: All the compounds were isolated from the Scutellaria barbata were showed significant anti-H. pylori activity; in addition, these compounds can decrease the MIC of Ampicillin (0.24 0.03 μg/mL). Key words Scutellaria barbata D. Don; Helicobacter pylori; Flavonoids 幽门螺杆菌（Helicobacter pylori）是寄居于胃及十二指肠中的一种微需氧，具有鞭毛的螺旋形革兰氏阴性菌。研究证据表明，H. pylori 与大多数胃及十二指肠疾病有关，包括：慢性胃及十二指肠炎症、胃及十二指肠溃疡、胃粘膜相关淋巴样组织淋巴瘤(MALTOMA)等；并且人群感染 H. pylori 的几率特别高，几乎一半以上的人群都有 H. pylori 感染，在发展中国家 H. pylori 感染尤为严重[1-4]。消除 H. pylori 能够促进溃疡愈合、阻止消化道溃疡复发、减小高危人群胃癌的发生几率[5]。 很多短程的药物被用于根除 H. pylori 的感染，包括抗生素、次杨酸铋制剂、质子泵阻断剂以及H2受体阻断剂以及