鉴别蜂蜜掺假的有效方法（An effective method for identification of adulteration in honey）.docVIP

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2017-08-16 发布于河南
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鉴别蜂蜜掺假的有效方法（An effective method for identification of adulteration in honey）.doc

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鉴别蜂蜜掺假的有效方法（An effective method for identification of adulteration in honey）

鉴别蜂蜜掺假的有效方法（An effective method for identification of adulteration in honey） Honey is the honey produced by bees from flowers of flowering plants and made from honey in the hive. Its main components are carbohydrates, of which 60% to 80% are easily absorbed glucose and fructose. In addition, it also contains various vitamins, minerals, flavonoids and amino acids. Firstly, the methods and advantages and disadvantages of honey adulteration were determined: (1) C- stable isotope analysis (method of stable carbon isotope ratio method, determination of sugar content of GB/T 18932.1-2002 -4 plant in honey carbon OAC- method 998.12), isotope contain sugar and protein of pure honey in value is the same, when doping fraud is only used to add sugar. If you add sugar to C4 plant (sugar cane sugar, corn syrup), because of significant differences in isotope sugar and protein of C4 plants and C3 plants derived from sugar honey value, thus adding sugar, honey sugar isotope values changed, and honey protein isotope values were not change, so will the C12 and C13 isotopes and honey protein of the two kinds of honey compared with the isotope method, to determine whether it contains C4 honey sugar, C4 sugar in honey share higher protein and honey value between the more negative. But because the shift in isotope relations is significantly smaller, the method can only be used to prove that more than 7% of the honey is added. Moreover, there is adulteration in the sugar of C3 plants (rice syrup and beet syrup), which can not be identified by isotopic methods. (2) high performance liquid chromatography refractive index method (HPLC-RID) method for the determination of GB-T 18932-22-2003 liquid fructose, glucose, sucrose, maltose content chromatography refractive index detection method, by means of high performance liquid with refractive index detector recognition chromatography can be fructose, glucose, sucrose and maltose were separation and direct determination of content of these four