煎煮时间对生大黄中五种游离蒽醌的影响（Effect of decocting time on five kinds of free anthraquinone in Rhubarb）.docVIP

煎煮时间对生大黄中五种游离蒽醌的影响（Effect of decocting time on five kinds of free anthraquinone in Rhubarb） Effect of decocting time on five kinds of free anthraquinone in Rhubarb Update Date: 01-24 [Abstract] [Objective] the relationship between total amount of five kinds of free anthraquinone (aloe emodin, rhein, emodin, chrysophanol, emodin, etc.) and decocting time was studied. Methods using HPLC, using methanol - 0.1% phosphoric acid (85: 15) as mobile phase, detection wavelength: 254nm; flow rate: 1.0ml/min, column temperature: room temperature, after the detection of rhubarb boil every 2 minutes when five anthraquinones by water decoction in the samples of the 20min content. After boiling, the total content of five anthraquinones increased after decreased. Conclusion the use of force to continue after boiling boiling about 20min when the total content of five kinds of anthraquinone in the highest. Raw rhubarb; free anthraquinone; high performance liquid chromatography (HPLC) Abstract: Objective To determine the relation between the total count of the five free anthraquinone (A loe emodin, Rhein, Emodin, Chrysophanol, Physcion) in Radix et Rhizoma Rhei and the decoction time. Method HPLC was used with the chromatographic conditions as follows:mobile phase:methanol 0.1% phosphonic acid solution = 85:15, detection wave length:254nm flow, rate:1.0 ml/min, column temperature:room temperature.The count of the five free anthraquinone in the decoction taken in every after the solution boiled 2 minutes was determined. Results During the after the solution was 22 minutes boiled, the total count took on a ascending tendency, afterwards it, took on a descending tendency. Conclus Ion] The, biggest, number, of, the, total, count, was, obtained, 20min,, after, the, solution, was, boiled. Key, words:Radix, et, Rhizoma, Rhei; Free Anthraquinone; HPLC Rhubarb (Rheum officnale Baill) for Polygonum cuspidatum (Rheum palmatumd), rhubarb (Rheum officinale Baill) and r.tanguticum (Rheum tanguticum