科学仪器学 激光共聚焦（Scientific instrument confocal laser）.docVIP

科学仪器学 激光共聚焦（Scientific instrument confocal laser） Characteristics and applications of laser scanning confocal microscope LSCM laser scanning confocal microscope (Laser Scanning Confocal Microscope) is a kind of micro equipment which has been put into practical application in 80s of last century. Compared with the ordinary optical microscope, LSCM has higher resolution and magnification, and can scan the observation samples, 3D reconstruction and measurement analysis of the sample is realized; compared with the electron microscope, real-time dynamic changes of LSCM can be observed and the cell morphology such as Ca2+, pH and membrane potential and other physiological signals at the subcellular level the. Therefore, the development of this product is an epoch-making significant progress in the development of micro imaging technology. LSCM has a wide range of applications in the fields of morphology, molecular biology, neurology, pharmacology and genetics, as well as in the fields of materials science, geology, water conservancy and other industrial engineering fields. The laser scanning confocal microscope and industrial laser scanning confocal microscope can be divided into biological laser scanning confocal microscope and laser scanning confocal microscope. The basic principle and structural features of a.LSCM Halogen light source using ordinary optical microscope for hybrid optical imaging, wide spectral range, each sample according to spot are subject to interference caused by chromatic aberration and light scattering and diffraction, affect the imaging quality. While the LSCM structure adopts precision confocal spatial filtering, the unique design of the formation of the object image conjugate, the laser lens focal plane pinhole formation point light source scanning the sample, detection pinhole at the focal plane of the lens to measure through spatial filtering, effectively inhibit the same focal plane non measuring the formation of spurious fluorescence and sa